FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s85.352000000d8588.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s85.352000000d8588.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4350708
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA446681.0266834731266727No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA87120.20024327075041579No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA71960.16539836734618826No Hit
GTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGGTTA57440.13202448888778562No Hit
GGTACTATGATTTCACCGAGTCTCTCGTTGAGACAGTGCCCAAATCGTTA50420.11588918401326866No Hit
GAATAGGCCCAAGCGACTGTTTATCAAAAACACAGGTCTCTGCTAAACCG43510.10000671155131532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT241400.053.4950142
CCGATCT245400.051.945664
CGATCTG102050.051.2407725
TCCGATC257750.049.991073
CGATCTA49550.047.2546545
CTTCCGA313750.042.9605671
GATCTGG46500.039.1193966
CGATCTC79800.037.4954835
CGATCTT62800.037.133275
GATCTGC40100.036.4798166
GATCTGA36300.033.6258136
GATCTAG22750.033.4029586
GATCTAA16350.028.7583396
ATCTGGT16650.028.5254217
GATCTCA28450.027.8792156
TCTTCCG150500.027.7391855
GTGCTCT153650.027.077771
TGCTCTT155750.026.529712
GATCTTA13400.026.2284896
GATCTTT36700.025.3650866
Produced by FastQC (version 0.11.2)