FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s84.352000000d857b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s84.352000000d857b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT60.45627376425855515Illumina RNA PCR Primer (95% over 24bp)
CATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTCGCCGT40.3041825095057034Illumina Single End PCR Primer 1 (100% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3041825095057034No Hit
CCCTGGAAAGGGTGGCCAGAGCAGGTGATAGCCCTGTAACCGACCGAGCG30.22813688212927757No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG30.22813688212927757No Hit
TCGTTACGCCTTTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCT20.1520912547528517No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGT20.1520912547528517No Hit
ATACACATCTGACGCTGCCGACGATACCCCTTGTGTAGATCTCGGTGGTC20.1520912547528517No Hit
CTATAATTTCACCGAGTCTCTCGTTGAGACAGTGCCCAGATCGTTGCACC20.1520912547528517No Hit
CAATAATTCCGGATAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGC20.1520912547528517No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC20.1520912547528517No Hit
CATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT20.1520912547528517Illumina RNA PCR Primer (100% over 25bp)
GTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTT20.1520912547528517No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG20.1520912547528517No Hit
GTATTTAGGCTTGGGAGATGGTCCTCCCGGCTTCCGACGGCATATCACGT20.1520912547528517No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)