FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s83.352000000d8552.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s83.352000000d8552.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6285214
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA345220.5492573522556272No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT85230.1356039746618015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACGT82550.039.0090458
GGAGACG93400.037.6302837
GGGAGAC96300.037.5818256
CCGATCT197650.036.5002944
TCCGATC206000.035.3666153
TTCCGAT207550.034.96522
AGACGTG89300.033.7202229
AGGGAGA51100.029.9277325
CTTCCGA273100.029.5987421
TGGGAGA47150.023.8729175
CGATCTG123300.022.9188465
TCCGAAC36200.022.6972333
GATCTAG23750.021.997756
CGATCTA68800.021.5379815
TAGGACT52200.020.9263114
CAGGGAG43050.020.8508634
CTAGGAC54300.019.9420783
TCTAGGA58150.019.6018432
ATCTAGG55500.019.596471
AGGACTA56500.019.0816635
Produced by FastQC (version 0.11.2)