FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s82.352000000d8538.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s82.352000000d8538.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.7292616226071102No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.27347310847766637No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG30.13673655423883319No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC30.13673655423883319No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13673655423883319No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCC352.395118E-667.857158
GCTGCCG352.395118E-667.857159
ACGCTGC300.00898901147.57
CTGACGC300.00898901147.54
TCTGACG300.00898901147.53
CCCTCCG150.00964477847.522-23
TGACGCT300.00898901147.55
TCCCTCC150.00964477847.520-21
TGCCGAC350.007510529427.14285910-11
CTGCCGA350.007510529427.14285910-11
Produced by FastQC (version 0.11.2)