FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s81.352000000d8501.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s81.352000000d8501.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3737
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.6422263848006422No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.2675943270002676No Hit
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT80.21407546160021407Illumina RNA PCR Primer (95% over 24bp)
CATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT60.16055659620016055Illumina RNA PCR Primer (100% over 25bp)
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC60.16055659620016055No Hit
CATCTGACGCTGCCGACGATACCCCTTGTGTAGATCTCGGTGGTCGCCGT40.10703773080010703Illumina Single End PCR Primer 1 (100% over 23bp)

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)