FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s80.352000000d84f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s80.352000000d84f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAATACTTGTATTTTTTCATATGTTCCTCCTATTTAAAAACCTGCAT21.4598540145985401No Hit
AGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGC10.7299270072992701No Hit
CTATTACTTTTTGAAGGATTTAGGAAAATTAATTTTTGGATTAATTTTCC10.7299270072992701No Hit
ATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAA10.7299270072992701No Hit
GAACGAAGGTTAGTGTATCGAAAACGATTAGATACCGTTGTAGTCTTAAC10.7299270072992701No Hit
GCCTCTCACGGGCACCTCCCCCAGGCTGGCTCACTGGAGCGGAACCGCGT10.7299270072992701No Hit
TTATGCTACCTTTGCACAGTCAAAATACTGCGGCAATTTAAAATTTTCAG10.7299270072992701No Hit
CTCTAAAAGTGTAACTACATTTAAAAATAAAGCGTTGGCAGCGATTGTTC10.7299270072992701No Hit
CATCTTTGGCAAATGCTTTCGCTTAAGTTAGTCTTACGACGGTCCAAGAA10.7299270072992701No Hit
CTCGTGCATGTTGGGTCGGGCTCCTCCGGCGGGGCTGGAAGCGGAGTAGG10.7299270072992701No Hit
GTCCTAATGTTATTCCAGCTAATATATTCGGGCCTATGGCCTGCTTTGAA10.7299270072992701No Hit
GGCCCATGTTCATTCCCTTCCAGCGAGTGCCCATTCCCTTCGGACGCATT10.7299270072992701No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAA10.7299270072992701No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA10.7299270072992701No Hit
GTCCTTCTTCCAAGTGCCTCCCCCCTTCTACACTCAACAAATATACATAA10.7299270072992701No Hit
TCTACGACCATGTGAGCAAGCACTTGACCACGGATGCCGTGTGCCATGTG10.7299270072992701No Hit
ACCCATCGTAATAATCTAATCCCAGTATCGATTATTTCGCGATTAACAAC10.7299270072992701No Hit
TTATCCTTCCTTGCAACACACTTGCTCTCCAAGCAGCAGGAGAACCAGGG10.7299270072992701No Hit
ATAGCTTTTTTTTTCACATTCTTGTGAAATTATTTCCTTCTCAGAATTTG10.7299270072992701No Hit
AACCCAAGCTCTCTCTATCTCTTAATCTCTCTGTGCTGTGTGTCTCCGTG10.7299270072992701No Hit
GATGAGTAGAATGCATGCAGTGCTTGCAGTCATTCTGTCCAAGGATCTGT10.7299270072992701No Hit
TACCGTGCCCATGGTCGCATCAATCCCTACATGTCGTCGCCCTGTCTCTT10.7299270072992701No Hit
GAAAACAACAGCGAAAAGCGAAAATCCTTGAGCACGAAAACGACTCCTGC10.7299270072992701No Hit
CTGGTGGAGTGGGAAAATCTCCCCTCTGCCTATGTATATCCTCACTGATC10.7299270072992701No Hit
ACACTTTCTAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGGTGGT10.7299270072992701No Hit
GTATTCAGGAGACCTATCTCATGTGCAAAGACACACATAGGCTCAAAATA10.7299270072992701No Hit
TCAATCAATCAATCAATCAATCAATCAACAACGATCCGTGGATATATGCA10.7299270072992701No Hit
GCTTTTATTAGGCTAAAAACCAAGCGATCGCAAGATCGTTATATTGGTTG10.7299270072992701No Hit
TCTGAAACGAAAGCCAATCGGTCGTGATATTCCCACACGCGCAGCTGCTT10.7299270072992701No Hit
GAAGTGAGGAAAGGTTACACGATTACAGCAATTGAACGTGGGTTAGCCGA10.7299270072992701No Hit
AAAAAAAGATAAAAGAGAAAAAAGACAAGCAAATATACAAGAAAACAAAA10.7299270072992701No Hit
GATCAACGCATCGATCTTGTACAGCTCCGGGTACACGTACTTCATTAGCT10.7299270072992701No Hit
GCTTCAGTCCCAGGAGGAGAGAAGGTGAGTTTCCACATTTTACCTTCTGG10.7299270072992701No Hit
GATCAAGAGGTAATGATTAATAGAAGCAGTTTGGGGGCATTAGTATTACC10.7299270072992701No Hit
TATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTA10.7299270072992701No Hit
GTACCGTACTGTATCTTATGCAATCTGCACTTTTCAGGCCGAAGTTTCTG10.7299270072992701No Hit
GACATAAGGGGGCAACAAATTGTCAACCCGTCTATTTAGCAGGTCAAGGT10.7299270072992701No Hit
ACCTTGACCTGCTAAATAGACGGGTTGACAATTTGTTGGCCCCTTGTCTT10.7299270072992701No Hit
ATGCCGGGGTTCACGGTGTAAGCGGTCACGCCGGTAATGGGGGCCAGTTT10.7299270072992701No Hit
CTCGTACACCATTAGGTTTCCTCAGGCTATGGTGACAATGCTCAGCACCT10.7299270072992701No Hit
GGACGCACAGGACCTTGGGGCCTGCAGTGTACCAGGCCTTGGGGTTGGGT10.7299270072992701No Hit
CCCTCAATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCA10.7299270072992701No Hit
CTTTTGCACATACGAAAATTGAAGGCCGAACGCTGGGCGCACAATCAGGG10.7299270072992701No Hit
CCCTCTATGATGCCCTTTTCCAAGGGACTTAGGCATGGTCAGAAGCGGAA10.7299270072992701No Hit
ATCTCTCCGATGGATCCACGCCCGGGCCCAGATCAACTGCTCAGGCACAG10.7299270072992701No Hit
CTATGATGCCCTTTTCCAAGGGACTTAGGCATGGTCCGAAGCGGAAAACA10.7299270072992701No Hit
TGGCCGTTCTTAGTTCGTGGAGTGATTTGTCTGGTTAATTCCGATAACGA10.7299270072992701No Hit
TTAGTGGAGACAGTAGGCCACGATTAGGGTAACCACCGTGGAGATGAAGC10.7299270072992701No Hit
ACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTA10.7299270072992701No Hit
CAGGTATAGGCTTCACCATCATCATTGCAGAGTGTCTGTTGTCGAATACC10.7299270072992701No Hit
GCGATGTATGACATTCCGTATCCAAAGAATTATTTCTAAGGTTGCGGCAA10.7299270072992701No Hit
ATTTTAATTTTTGCCGCCGACGGGTTGACCAAGCGAAGCGCGGTAGGTTT10.7299270072992701No Hit
GTGTTAGGTTCCCATCGTCGTCGTCGTTTGCCAATCCGTTTGTGGGCTTC10.7299270072992701No Hit
GGCTGGGCGTGATTCTATCCTTCTTGGCCACGATCCTCTTGTCCTTGGCC10.7299270072992701No Hit
GGATGGCCCGTTTCCTCTTGCATCGCATCTCCGAGGAGTTTGGCATTGTG10.7299270072992701No Hit
ATATTTAGCCTTAGATGGCATTTACCACCCATTTTGAGCTGCATTCCCAA10.7299270072992701No Hit
TTACAGGGCCTCGGATATGAGTCATGTATTGTTATTTTTCGTCACTACCT10.7299270072992701No Hit
ATTTAGTGGTATTAAGACATTACTCTATGTAGCGTGGAGAGTGTCTGGGC10.7299270072992701No Hit
TCCTGAAGCTACTAAGGCCCGTATCGTCATGCCCGATTTCTTCCGAGGCA10.7299270072992701No Hit
CCCAACGGCCACTCGATCGGCATCGAAATCTGCGCCGACGGGGGGCTCAC10.7299270072992701No Hit
GTTTAGGTATCCGCTTTTCGTTTGCCCTTACTTCGAGTGGTTGATCATAG10.7299270072992701No Hit
ATGGTACGCTGGACGCTGTAGGATACCCTCGCTTTCCTGCTCCTGTTGAG10.7299270072992701No Hit
GTGTCTCTGTTATGTGAGGACAAGAAGAGCCAGAATGCTTGAGGCCATGT10.7299270072992701No Hit
TTACACTTCTGACACTGCCGACGAAGAGGGATACCCTCGCTTTCCTGCTC10.7299270072992701No Hit
TGATAATCCCGTACATGGCATGGAGCGCCCGAAGCTTTGTGATACACTTT10.7299270072992701No Hit
GCCGTGGACTACGCCCAGAAGCGACAGGCCTTCGGCAAGCCCATTGCCAA10.7299270072992701No Hit
GATCAGGTCAAATAGTTTCCCTAGGGCTAAAGTCTCTTATTAGAGCAATT10.7299270072992701No Hit
GTCAAAAGCCTCGCAAAAAAGCTTAGCTGATGAAATTGATGGCTATTTTG10.7299270072992701No Hit
TTGTAATCTCAAGAAGTGTTTTCCGCTTCGGACCATGCCTAAGTCCCTTG10.7299270072992701No Hit
CACCAGACTTGCCCTCCAATTGGTCCTTGTTAAAGGATTTAAAGTGTACT10.7299270072992701No Hit
GTGATAATCCCGTACATGGCATGGAGCGCCAGAAGCTTTGTGATACACTT10.7299270072992701No Hit
ATTGAAAGCTGGCGTCTTGCGTCCGCATTGTAATCTCAAGAAGTGTTTTC10.7299270072992701No Hit
GCTGAAAGTGGAAGAAGCAACTGCAGCAGCCGCATAAAATTCGAGAAGAA10.7299270072992701No Hit
CACCTACAGATACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTT10.7299270072992701No Hit
TATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGA10.7299270072992701No Hit
GTTTTTATTTTTGTTTGCTTTTTTTTTTCATTTGCACAATCCACCCACAG10.7299270072992701No Hit
CTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATG10.7299270072992701No Hit
GTCGAGGATTACATTTGCGTGAAATCAGAGACAATCGAACGCACAGGACA10.7299270072992701No Hit
TAGTAATTCCTGCTTTATCAAGATAATTTTTCGACTCATCAGAAATATCC10.7299270072992701No Hit
GATTGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAA10.7299270072992701No Hit
GGTAATGATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGA10.7299270072992701No Hit
GTCCTGAATGCTGTTTGCTGGGCGAGGTTCCAGTGCCAGTTGCTCGCAGT10.7299270072992701No Hit
GGTAGTCCTCAGTCTTACCAGGTGCTCTTTGCATATTCACACGTGACCCC10.7299270072992701No Hit
GCTGACAAGGCCAAGGAAGACCGGTCTGAAAGGTGATCGGTGCATGACCG10.7299270072992701No Hit
CCATAAACGATGCCAGCTAGCAATTGGGTGTAGCTACTTTTATGGCTCTC10.7299270072992701No Hit
GAGCTCCCAAATTCTAGAAACGCGTCCTACCCGCCCACATTTATAGCGAG10.7299270072992701No Hit
GATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAA10.7299270072992701No Hit
TATTCATATAGGCCACATATCCCAGGACACAGGTCAGCGCGATTATGACG10.7299270072992701No Hit
ACAATAACAACAGCAGCAGCAGCAACAACAATAACAATAACAACAATACC10.7299270072992701No Hit
GTGTGGACCATTGTGATTGCTATAGGTGTCCAGAATGATGGCCAGTCCAG10.7299270072992701No Hit
GCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGA10.7299270072992701No Hit
CAATTTGCACCGGGCGCCTTTCCCTTTCCTCCGCTCTCCACGCCCTGTCT10.7299270072992701No Hit
ACGGTCTCCTGAACTTTAACCTGACTATTCCACTGCCACAAGTGAACGGA10.7299270072992701No Hit
CCCAGATTCAGATCCAGCCCATGCCCTTCGGAATGGCCCTGCAGCGCGTT10.7299270072992701No Hit
CACGATCAGCCGCCTGACCCCTGTGTTGCCGATAGTGGACTAGTCCACAC10.7299270072992701No Hit
ATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGT10.7299270072992701No Hit
ATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGTATCCAAC10.7299270072992701No Hit
CTGCACTAGAGGCCGTTCCACCCAGCTTCACAGCCAAGGCTTCCATACGG10.7299270072992701No Hit
AGTGTAGATCTTGGTGGCCGCAGCAAATTTTAAAATGAGAACCTTGAAGA10.7299270072992701No Hit
GTCCGCTCCGGCGCTCGTCGCTGCGGTGAAGCTTGCGTTTATGGTACGCT10.7299270072992701No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAGCCCAAAAGCTT10.7299270072992701No Hit
GTACACTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTCTGTC10.7299270072992701No Hit
ACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATA10.7299270072992701No Hit
GCTTATTACCAGCGCTAAAGATTACTATTACCTTTTTAAGGATTTAAGGA10.7299270072992701No Hit
CAAAGGCCCTAACCACCTAACCCTTAGAGCCAATCCTTATCCCGAAGTTA10.7299270072992701No Hit
ACGCGTGCCTTCGCACTAAAGCTCCAGATGCGCGTGGTGCTGCCGATCCC10.7299270072992701No Hit
GTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGT10.7299270072992701No Hit
AAAAAGAGACGCGAGAAATCTCAACGAGAACAGCAAAATCAAATTCACCA10.7299270072992701No Hit
AGATGGCATTTACCACCCATTTTGAGCTGCATTCCCAAACAACTCGACTC10.7299270072992701No Hit
GCACCAGGCGCCCATTCTTTAGCTCCCCCCACCCGCGTCTCGTAATATCA10.7299270072992701No Hit
GTCCGAAGCGGAAAACACTTCTTGAGATTACAATGTGGACGCAAAAGACG10.7299270072992701No Hit
GCTAAAATGTGCTCAAGTCTGGCCCATTTAGGGGTGGCTCCATGGAAGCG10.7299270072992701No Hit
ATGCAGAGACCCAGCAGAAGAACGAGGTGATTCAGCAGCAGGCTCAGCAG10.7299270072992701No Hit
AACGCGGAATAGCAAAGCCTCTACGCGATTTCATAGTGGAGGCCTCCAGC10.7299270072992701No Hit
CATAGAGGGTGATAATCCCGTACATGGCATGGAGCCCCCGAAGCTTTGTG10.7299270072992701No Hit
AAGTAGCTACACCCAATTGCTAGCTGGCATCGTTTATGGTTAGAACTAGG10.7299270072992701No Hit
ATGTACACGCGCAGCAACCCGTGTGAACTGCGTTCGCAGTCCACGAGTTC10.7299270072992701No Hit
GCTAACAGCAACCACAGCAAGGACGACCACAATGGAACCCTCCTCGGAAT10.7299270072992701No Hit
CCGCAAGGAGGGAATGTAGCGAAATGATCTGCTTTCCGCCAACGTGAATA10.7299270072992701No Hit
CTCTTCCGATCTGACTGGGGATTCTGGTTGATCCTGCCAGTAGTTATATG10.7299270072992701No Hit
CCTTTCTTCCGTTTCGTCAAAGTGTGCTCAATTAATTATTTGTCATTGGT10.7299270072992701No Hit
TTTATTATCCATACGATTAGCGCCGTAAATTTCTAGCCAATCGCACTTTG10.7299270072992701No Hit
GAACAGGGCGTTCAGTTCGTGGCTTTAGACCAGAAACCGAGTGATCTAGC10.7299270072992701No Hit
ACCCTCGTGGGTGATGCCCACACCGAGGCGAACAATGCCAGCGTTGTTGA10.7299270072992701No Hit
TCTTTGAGCAAATCGCCTGCCTCCTCGTCTAACGGCCCGGCGTCTTGGTA10.7299270072992701No Hit
ATATCGGGGATTACGTTTTGGGTCTCGAATGCCCAATAGTGGCTGCCAAC10.7299270072992701No Hit
TTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGA10.7299270072992701No Hit
CTCTTTAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTCTCTCAATCC10.7299270072992701No Hit
ATCATGGCGTGGGCATCTTTCTTGACGCGCGGCGGAATATCTCAGTTGAC10.7299270072992701No Hit
CGCATCAGCTGTTTGTGCTAATTTCGTTCGCTCTGCCACGGCTGCTGTTG10.7299270072992701No Hit
CCCAGCAAGAACCATACGACCAATATCATGTAAATAGTCACGCAAAGCAT10.7299270072992701No Hit
GTACAAAGTACCTGGTCTAACACCCGGCCGTGGTCAGAACATCTACAACT10.7299270072992701No Hit
GCACTGCAATGCGGAGGAGGCAAAGTACTGGCAGCATTTTCGAAAATCCC10.7299270072992701No Hit
GTATCATCGTCGCCAACGTGCCCGAGGGTCTGCTGGCCACCGTAACTGTG10.7299270072992701No Hit
CCCGGCGTACGGGGAAGGACGTCAATAGTCACACAGTCCTTGACGGTATA10.7299270072992701No Hit
AATTAAAATTTTTACCGCTTCGGCGTTATAACCTCACACTCAATCTTTTA10.7299270072992701No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers