FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s8.352000000d7c0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s8.352000000d7c0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAAGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTG14.545454545454546No Hit
ACACGCAAGGTAAACGCGAACAATTCAGCGGCTTTAACCGGACGCTCGAC14.545454545454546No Hit
GTAAAAAATATCCCCACGGAAAAGCATAGTGCTATCGTGGGGATTTTTTC14.545454545454546No Hit
CCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCT14.545454545454546No Hit
ATCACGTTCTTGGTCAGTATGCAAATTAGCATAAGCAGCTTGCAGACCCA14.545454545454546No Hit
GCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGT14.545454545454546No Hit
TTACCTAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGCAG14.545454545454546No Hit
ATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTTCTCACTTC14.545454545454546No Hit
ATTCTGGTGGAGCTCCCCAAGGGGGACTGGGCCTACCATGGTGCGGCAGT14.545454545454546No Hit
CCTTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCGTCGCCGG14.545454545454546No Hit
TTTGACTTATACCGATATTGCTGGCGACCCTGTTTTGTATGGCAACTTGC14.545454545454546No Hit
CTGTGTACGTTTTATATTGCATCCCGTTTCCAACGAAATCCTCAAAGCGA14.545454545454546No Hit
GCTTAGCGCGAGTCGCACAAATCGCACCAGCTCACTCCGATGTTGATTTG14.545454545454546No Hit
GTCGGCTACAGTAACTTTTCCCAGCCTCAATCTCATCTCTCTTTTTGCGT14.545454545454546No Hit
AAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTATTGAGAAGTT14.545454545454546No Hit
AAGTGCCAGCCTGCAACGTACCTTCAAGAAGTCCTTTACCAGCTTTAGCC14.545454545454546No Hit
TGACTATAATTTGGCTTATGTGCAGTAAATTATACTCTTGAATATGTTAG14.545454545454546No Hit
CAACAAATATTGAGTGATAAATGAGTAAATAATAATTGCCCCATTTGGGG14.545454545454546No Hit
CAAGAACGTGATTACTTCATGCAGCGTTACCATGATGTTATTTCTTCATT14.545454545454546No Hit
CGACTCATTTCACAATCACTTCGTCACCGCAACCTTTCCAGAAACTACTC14.545454545454546No Hit
CGTCTGATCGGAAGAGCACACGTCTGATCGGAAGAGCACACGTCTGATCG14.545454545454546Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
GGACAAGGGAGCTCTCTTGGTCCTCATTAAAAGACACTAATTCTGTTTAT14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)