FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s76.352000000d8489.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s76.352000000d8489.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTACAAACTCTCGTGGTGTGACGGGCGGTGTGTACAAGACCCGGGAA30.2840909090909091No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG30.2840909090909091No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA30.2840909090909091No Hit
CCCATAAAGTTGTTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATG20.1893939393939394No Hit
TACCTAATCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATA20.1893939393939394No Hit
CTTCAAATCTCCAACGCCCGCGACGGATAGGGACCGAACTGTCTCACGAC20.1893939393939394No Hit
CCATCTCCCAAGCCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACC20.1893939393939394No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA20.1893939393939394No Hit
GTGTAAAGGCAGAAGGGAGCTTGACTGCGAGACAGACAAGTCGAGCAGGG20.1893939393939394No Hit
ATTCAGTTGGGCACTCTAAGTTGACTGCCGGTGACAAACCGGAGGAAGGT20.1893939393939394No Hit
GTTACGGGGCCATTTTGCCGAGTTCCTTAACGAGAGTTCACTCGCTCACC20.1893939393939394No Hit
ACTTGTAAGCATACGGTTTCAGGTGCTCTTTCACTCCCCTCCCGGGGTTC20.1893939393939394No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)