FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s75.352000000d846f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s75.352000000d846f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5644621
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT106040.1878602655519299No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104020.1842816373322496No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG86080.15249916690598003No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT85730.15187910756098594No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA84120.14902683457401303No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC72420.1282991364699242No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA67190.11903367825758364No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC66020.11696090844717476No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT65190.11549048200047445No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT64540.11433894321691396No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG63540.11256734508835935No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA60000.10629588771327607No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG59470.10535694070514211No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC71750.041.701116
TTCCGAT158650.035.5323752
CCGATCT159100.034.9841234
TCCGATC169150.033.1301653
GAGACGT85200.032.498968
CTTCCGA191650.031.0743661
AGGGAGA46400.031.0126025
GGAGACG95450.030.9990797
AGACGTG86550.028.9741869
TGGGAGA35200.028.6026845
CAGGGAG32450.022.538224
AGACGTT12400.020.6831389
CGATCTC63900.019.9924035
GATCTAG15550.019.8523526
ATCTAGG66450.019.368141
TAGGACT70650.019.2922694
GTCTTAC51400.019.2182481
CGATCTG123900.019.0501615
CTTGTCT45150.018.934739
ATATAAG37950.018.7712442
Produced by FastQC (version 0.11.2)