FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s74.352000000d8453.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s74.352000000d8453.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7253513
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA222690.3070098585333755No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT110040.1517058010373732No Hit
CCCCAATATTTAGCCTTAGATGGCATTTACCACCCATTTTGAGCTGCATT97080.13383859655314603No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT81970.11300731107809416No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG80930.11157352306392777No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC73490.10131642419335295No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA72890.10048923880056464No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT168500.040.38794
TCCGATC170900.040.3481453
TTCCGAT173650.039.6818242
CTTCCGA219400.034.0263181
GGGAGAC57150.032.6582766
GAGACGT53750.031.1019158
GGAGACG67050.026.4908437
AGACGTG60750.023.8438579
CGATCTC66000.023.4574955
GATCTAG20700.022.2545266
CGATCTG127600.021.2889045
TCTTCCG128200.020.4483875
GTATTAT19300.019.9308911
ACTGTAC31150.019.3617533
CGATCTA72450.019.2060135
AGGGAGA39800.019.0917155
TGGGAGA37550.018.4650695
TCTAGGA69250.018.2415122
CCCAATA76050.018.2340282
CTAGGAC68100.017.921943
Produced by FastQC (version 0.11.2)