FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s72.352000000d842c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s72.352000000d842c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTACAACAACTATTTCAGAGCGCCGTGGAGGCCTAACCGTACCGAGTC14.0No Hit
ATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTT14.0No Hit
TGTTTATCCTTTGGATGGTCGCCATGATGGTGGTTATTATACCGTCAAGG14.0No Hit
ATACCACTGATTCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAA14.0No Hit
TGCTAGTTTGACGGTTAAAGCTGGTAATGGTGGTTTTCTTCATTGCATTC14.0No Hit
GGTTGACTTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTC14.0No Hit
ACTTTGTAGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCC14.0No Hit
CATATTTAGCTCTCTACGAAATTTCTGGTGAATCTAAAAGTTGTCGCGAT14.0No Hit
AGTGTTCACGATTGCTGCAGCCCTCCACTATGAAATCGCGTAGAGGCTTT14.0No Hit
GAGTGGTCGGCAGATTGCGATAAACGGTCACATTAAATTTAACCTGACTA14.0No Hit
CAAGCAACAGCCGGATTCCGACAATATGCGCCAAATGCTTACTCAAGCTC14.0No Hit
CTGAGTATGGTACAGCTAATGGCCGTCTTCATTTCCATGCGGTGCACTTT14.0No Hit
GAAAGAAGACCCTGTTGAGCTTGACTCTAGTTTGACATTGTGAAGAGACA14.0No Hit
GTAGTAATTCCTGCTTTATCAAGATAATTTTTCGACTCATCAGAAATATC14.0No Hit
ACTTATCAGAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAGAAGT14.0No Hit
GAGGCCTCCAGCAATCTTGAACACTCATCCTTAATACCTTTCTTTTTGGG14.0No Hit
TGTTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTG14.0No Hit
TCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGAGTC14.0No Hit
ATTGTGCAGTTGCATTTTAGTAAGCTCTTTTTGATTCTCAGATCCGGCGT14.0No Hit
CCGCGCTGCAAACGGAGACTACAAAATAGACTGCTGGCATCCCGTCGGCG14.0No Hit
CTGGTTGCACGGCCTCGCGTCGTAACCCAGCTTGGTAACTTGGATTAAGC14.0No Hit
CTCATTTTCCGCCAGCAGTCCACTTCGATTTAATTCGTAAACAAGCAGTA14.0No Hit
ACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTA14.0No Hit
AGCCAGCTTGCGGCAAAACTGAGTAACCGTCTTCTCGTTCTCTAAAAACC14.0No Hit
ATTAACAGTTGTCTCGTTCGATAAATTCCACTGTTTGTCCCCTTGGGGGC14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)