FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s71.352000000d83f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s71.352000000d83f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5422088
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA178580.3293565135792706No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT134820.24864959771955011No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG93910.17319895951522735No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT92730.17102267613509778No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA81330.14999756551350696No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC76730.14151374894690016No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC74970.13826776695619844No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA73430.1354275327143344No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT73390.1353537603963639No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT71620.13208933532616957No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG67340.12419569730332668No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA66150.1220009708437045No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG65750.12126324766399954No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA60970.11244745566652552No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA59740.11017895688893281No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA58050.10706207645467945No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA57560.10615836555954089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT174450.051.9988482
TCCGATC179400.050.3523143
CCGATCT177800.050.3246154
CTTCCGA208350.045.4302521
CGATCTC64900.032.6378485
CGATCTG142950.027.741745
TCTTCCG106250.027.043185
GCTCTTC110100.026.2700753
GTGCTCT118750.024.556481
TATGCTT42350.023.7746325
CTCTTCC127100.023.1300434
ATATAAG28000.023.0681212
TGCTCTT134350.021.7404632
CTTGTCT45100.021.1673689
CGATCTA74500.020.6547585
ATGCTTG46600.020.4858266
TAGGACT74450.020.4772514
ATATGCT49600.020.3952624
GATCTAG21700.020.135926
ATCTAGG77500.020.0390621
Produced by FastQC (version 0.11.2)