FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s70.352000000d83e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s70.352000000d83e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4498803
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA344430.765603650571052No Hit
GAATAATAAAATAGGACGTGCGGTTCTATTTTGTTGGTTTTTGGAATCGC63290.14068186582075276No Hit
GATTAATAGGGACAGTCGGGGGCATTAGTACTCACATGCTAGAGGTGAAA54420.12096551015903564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT170550.049.285054
TCCGATC174700.048.657953
TTCCGAT180500.047.30492
CGATCTG73200.045.0886655
CGATCTA34450.042.3197255
CTTCCGA231250.039.6135861
CGATCTC55050.034.764935
GATCTAG16350.033.403216
CGATCTT58650.030.4448265
ACTTTAG14000.029.5110661
GATCTGG41950.028.5283556
TTAGGGA16450.028.0026744
TGCTCTT123000.026.7946452
GTGCTCT125600.026.6180781
GATCTTT39600.026.1438456
GCTCTTC130300.025.2205943
TCTTCCG134900.024.8182325
ATCTAGC14350.024.4902277
GATCTGC44850.023.4011946
GATCTCA22800.023.3287416
Produced by FastQC (version 0.11.2)