FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s7.352000000d7be2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s7.352000000d7be2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG80.5878030859662012No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.5878030859662012No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT40.2939015429831006Illumina RNA PCR Primer (96% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2939015429831006No Hit
ATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTGGTC30.2204261572373255No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTTTAGATCTCGGTG20.1469507714915503No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1469507714915503No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC20.1469507714915503No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCC250.004005434557.08
GCTGCCG250.004005434557.09
ACGCTGC300.00824629247.57
GACGCTG300.00824629247.56
TGACGCT300.00824629247.55
CATCTGA403.6865685E-447.4999961
ATCTGAC403.6865685E-447.4999962
TGTAGAT250.001346812638.028-29
TGCCGAC350.00701665927.14285910-11
CTGCCGA350.00701665927.14285910-11
GCCGACG350.00701665927.14285912-13
CCGACGA350.00701665927.14285912-13
Produced by FastQC (version 0.11.2)