FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s68.352000000d83a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s68.352000000d83a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.3186743148502231No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.19120458891013384No Hit
GTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATGGT30.19120458891013384No Hit
CTTATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTG30.19120458891013384No Hit
ACATAACTCGTGTTGGGTTCCCCCATTCGGAAATCTCTGGATCACAGCTT20.12746972594008923No Hit
ATTACTAGCGATTCCGACTTCATGCAGGCGAGTTGCAGCCTGCAATCCGA20.12746972594008923No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12746972594008923No Hit
CACGTAGTTAGCCGTGGCTTTCTGATTAGGTACCGTCAAGACGCGCACAG20.12746972594008923No Hit
GTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGC20.12746972594008923No Hit
GTCCATCCCGGTCCTCTCGTACTAAGGATAGCTCTCTTCAAATCTCCAAC20.12746972594008923No Hit
GTAACCGACCGAGCGGTCGGCCCATGAGGATCCTGAGTACGGCGGCACAC20.12746972594008923No Hit
CTTTACGCCCAATAATTCCGGATAACGCTTGCCACCTACGTATTACCGCG20.12746972594008923No Hit
TTTCAGTGCTTCGCTTGAGCTAACACGTCCACTTAACCTTCCAGCACCGG20.12746972594008923No Hit
ATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTGGTC20.12746972594008923No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA20.12746972594008923No Hit
CATTAAAGCGGTACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTCGGT20.12746972594008923No Hit
ATCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTG20.12746972594008923No Hit
CGTTGAGACAGTGCCCAGATCGTTGCACCTTTCGTGCGGGTCGGAACTTA20.12746972594008923No Hit
ACTGTAGCCTGATATTGAAATTCGGCGCAGCTTGTACAGGATAGGTAGGA20.12746972594008923No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)