FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s67.352000000d838a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s67.352000000d838a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5225491
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT242180.46345884051852737No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA219400.4198648509776402No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT170370.3260363475891548No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA145600.27863410347467826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140720.26929526813843907No Hit
GTCTTAGACTTTTGCGGTTGTTGATTTTGCTTTCTTGTTTATTTAGCTGC75100.14371855199827155No Hit
GATATAACTTTGGTCTGATATTTGTTTTTTCTCATTTTGTCATTATCATT66150.1265909748959476No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT65680.12569153788610485No Hit
CTCTCGAATTGTGTCCGTTGGTAGCCGAAGTTACGATTGCAAAACTTTCG54830.1049279388290976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC53500.037.640436
GAGACGT49750.037.4227338
TTCCGAT121400.034.7402272
TCCGATC124350.033.7632943
CCGATCT122500.033.536554
AGACGTG52950.032.559919
GGAGACG63150.031.5125247
CTTCCGA154850.029.781491
ATAACTT40150.024.2497184
AGGGAGA38850.023.4719185
TTTGGTC41200.022.4791539
TGGGAGA31300.022.153735
GATATAA54600.021.9202881
TATAACT44400.021.6076133
AACTTTG47150.020.1460916
TAGGACT43750.019.7574864
GTATAAT15050.019.5656171
CTAGGAC43750.019.2146973
TCTAGGA46400.019.0385272
GTCCTAG33650.018.9128991
Produced by FastQC (version 0.11.2)