FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s66.352000000d837d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s66.352000000d837d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3571
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.3920470456454775No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.3920470456454775No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.3640436852422291No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.19602352282273874No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG50.14001680201624195No Hit
CTTATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTG50.14001680201624195No Hit
CATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTGGTCGCCGT50.14001680201624195Illumina Single End PCR Primer 1 (100% over 23bp)
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA40.11201344161299356No Hit
ATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTGGTC40.11201344161299356No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC300.00950399547.51
Produced by FastQC (version 0.11.2)