FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s65.352000000d8354.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s65.352000000d8354.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5628
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.6218905472636816No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.17768301350390903No Hit
CATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTGGTCGCCGT90.15991471215351813Illumina Single End PCR Primer 1 (100% over 23bp)
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG70.12437810945273632No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC60.10660980810234541No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT250.004764978357.02
TCCGATC250.004764978357.03
CCGATCT300.00980086347.54
CTTCCGA300.00980086347.51
ACATCTG607.281425E-539.5833368
GTAGATC250.001548439438.038-39
AGATCTC250.001548439438.040-41
TAGATCT250.001548439438.038-39
CACATCT651.1670383E-436.5384647
ACACATC550.002367436734.5454566
CATCTGA752.7074822E-431.6666689
ATCTCGG350.00804772827.14285542-43
GATCTCG350.00804772827.14285540-41
CCGACGA650.007117027418.26923220-21
TCTGACG800.001094108117.81253
Produced by FastQC (version 0.11.2)