FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s63.352000000d8310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s63.352000000d8310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAAGGTGCTGGAGAACCTTCCTAGCTTCTACTTTGAGAATTGTCGCG10.6211180124223602No Hit
GGGTAATTTACGCGCCTGCTCCCTTCCTTAGATGTGGTCCCGGGGTCGCA10.6211180124223602No Hit
CCTTCACAGGGAGAACGACAAGGAGTTTATGGTTGATGGGTGGGGGGAGC10.6211180124223602No Hit
GAACTATATGTAGCCAAACAAAGCAACCAAAAGGCCATTGCCCTAAAAGG10.6211180124223602No Hit
GCTAATCAGCGTGCACAAGAGGCCCGAAAAGCCGAAGGCTATCCAGCGCA10.6211180124223602No Hit
GCACAACTGCCTAATCCGTTTGGAGAGTTCATATACTGTCTCTTATACAC10.6211180124223602No Hit
CATCTGACGCTGCCGACGATACGCCTTGTGTAAATCTCGGTGCTCGTCAT10.6211180124223602No Hit
CGTCAGAAAAACAAAAAAAAAACAAACAAAAAAGCACCGGGGGGGCCACC10.6211180124223602No Hit
CTACACATCTAACGCTGCAGCCGATAAACCATATGTAGACCACGGTGGCC10.6211180124223602No Hit
CATGCACGCGTTTCTCTACATTTGCTCCCTGGTGGGCTCTTCCTCCGCCC10.6211180124223602No Hit
CCAAATCGTGTTGGTGGAGGACGAAAATACCTCTGATGTGCTGGCAGAGT10.6211180124223602No Hit
ATACACATCGGACGCTTCCTACGATACTCCTTGTATAGATCTCGGTAGAC10.6211180124223602No Hit
GAGTTCTGGTGGGCCCATCCTATTACTTTGAACACATATGAGATCGTAAA10.6211180124223602No Hit
CGCTACTGAAGCCAACATTACCGCGGCCTCCGCCCCCAAACTAACCCCCC10.6211180124223602No Hit
CTCTAAATTTTTGTCGTCACGCAAATCACATTGCATGACTCTACTGCCCC10.6211180124223602No Hit
CTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTA10.6211180124223602No Hit
GAAACGGGCGATAACCCATTAGCCACCCGACAGGTCAGAAACCTAGAAAC10.6211180124223602No Hit
CACTGGCGATGTAAATCAGTGTCCAGTTGGCCGTCATACACGACAGCTTT10.6211180124223602No Hit
AGGTGGGGATAAGATCGCCTTGTCGAGCTCACGTCTCGGCGTTAAAGTCA10.6211180124223602No Hit
GTGTATCAGCAGCGCCGCTGTCGCACCAGCAGCGCTGCCGACTCCACCAG10.6211180124223602No Hit
ATCCCCCCCGGTTATTTTTCCGCACCACCCCCCCGATCTTGGAGACCCAA10.6211180124223602No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA10.6211180124223602No Hit
CTTGCGATCAGTTCAGGGGACTTTCCGAGTTTCATTCGCCAAGCGAGCAC10.6211180124223602No Hit
CGTCCTACCCGCACCTTTTTGCTCGCCGGCCCCCCCAAAAAAAACCCACC10.6211180124223602No Hit
CAGAAAACACCCCCCGGGGCCCCCCCCTCCCACCCGAAGCCGTCTCTCAT10.6211180124223602No Hit
GAATCAAACAGTCTGGCTTTTTATTGTGGGCAAGTTGAACAATTAATAAA10.6211180124223602No Hit
GAGCAGCAGCCAAACATTACTTCAACATTGTTCACACCCGTGAGTTCCTC10.6211180124223602No Hit
AATTTCGCCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTA10.6211180124223602No Hit
CACTTGTACCGATAAGATTTCTTTGAACGTTCACACCTACTTAGATATCA10.6211180124223602No Hit
CATCTGACGCTGCCGACGAGACCCCTTGTCTAGATCTCGGTTGTCGCCGC10.6211180124223602No Hit
TCCCCGAGCTGGGAGGTAGTGACGAAAAATAACAATACCTGTCTCTTATA10.6211180124223602No Hit
TTCATATCCTCTCCACTGCTCTTCCTACCGCATTCAACTCTTGGCCTCCC10.6211180124223602No Hit
CCGACCTCCACGTTTCATACCTCTGCTTACTTCTCCCCACACCACATCCA10.6211180124223602No Hit
GTGTACACACAGCGCCCCCTAACCCTGTACCTCCTCCACTTCTCACGGTG10.6211180124223602No Hit
TATTAACCTTCACCTTCACAGCCACTTTTATTTCACCCCTTCTCCCTTTA10.6211180124223602No Hit
TCCCAGGTGGTCAACTCGCTCTTCCGCCAGCGCGCCGCCATAGAGAATAT10.6211180124223602No Hit
CATCTGACGCTGCCGACGATCCCCCTTGTGTAGATCCCGGTTGTTGGCGT10.6211180124223602No Hit
AAAAAAAAAAAAAAAAAAAAAAATATTAAATGTCACATTCCCTAAAGTAC10.6211180124223602No Hit
GGTCGACGCAACCACTGATCCCAGCCCTCTCTCATTCTTCTTTCCCCCAA10.6211180124223602No Hit
CATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTGGTCGCCGT10.6211180124223602Illumina Single End PCR Primer 1 (100% over 23bp)
TATCAAATGTCTGCCCTATTATCCTTTGATGGTAGTACCTAGGACTACCC10.6211180124223602No Hit
ATGGAGGGGTGAACTCCGCTCCAGTCGACAAACCCAGCTCCTCACCCTAT10.6211180124223602No Hit
CCGCTGGTTAGACCGCTCTGGCTGATGTATCCTCCCGATCCCGCCTGTCT10.6211180124223602No Hit
CTATTGCCGATCTTACTGTTTTCCTGTATTCCAACATCAACAAGACCCGT10.6211180124223602No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG10.6211180124223602No Hit
GCATGCTCTCTCGGCGGTGCCTCTGCAAGATACTTCTGCGACAAACGCAC10.6211180124223602No Hit
GTCAGAAACTCCGCACCAGGTCATGTTCTGAGCTAACTAAGACAGGATGT10.6211180124223602No Hit
AAAAAAAAAAAAAAAAAAAAAAACATATCACCACCCCCCCCCACACCCCC10.6211180124223602No Hit
CACGGAGGCGGATGACGTAGAGCGTCATCGCCAAGGTGACGTCGTACGTT10.6211180124223602No Hit
CAGAAGGGTGAGCCAGGACGTGCTGGAGCGACAGGTATGCCTGGTCGGCT10.6211180124223602No Hit
GTGCCACTTCCCGACGCCTTTAACCTGATTTTTTTCGCGGGTCTTAACAC10.6211180124223602No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA10.6211180124223602No Hit
CATCTGACGCTGCCGAAGATACGGCTTATGTGGATTGGGATGGACCCTGT10.6211180124223602No Hit
CAAAGCTAGAGATACGTGCGAGACACCCAGCGAGGTTACCAAAAAGTAAG10.6211180124223602No Hit
CATCTGACGCTGCCGACGATACTCCTGGTGTAGATCCCGGGGGTCGCCTT10.6211180124223602No Hit
CCACACCTCCGACCCCTCCTACTATACTCCTTGTGTAGTTCTCATTGGTT10.6211180124223602No Hit
ATATCGGTTCGCCTTTGCACCTCTAGCTTTCGTTCTTGCTTAATGAAAAC10.6211180124223602No Hit
TAATAGTACCGGCCAACAATAACACTCGTTTAAGAGCACTAATGCAGTCT10.6211180124223602No Hit
ATTAAAAACACAGCACTCTGCAAACACGAAAGTGGACGTATAAGGTGTGA10.6211180124223602No Hit
CTTCAGATCTCTCATGGGTTTTCAGCTTTACAAACATACTTCCAACGGAG10.6211180124223602No Hit
CTCACCCAACATCTTACCTCAGGTATCACTATCCTATAGAACACCAACCA10.6211180124223602No Hit
ATGTCAACCAGTATCAGTATGTCGCACACTTTTTCCCCCACTTACACCTA10.6211180124223602No Hit
CCTCCGCCACTGCACACTATACTCCCTTTTTATATCTCTGTGACTCCCAC10.6211180124223602No Hit
GTTGAGTCGTCGCCAGCGCTGACGCCGTCACAGTAGATCTCCTCGACGGC10.6211180124223602No Hit
GTCGATCGTTGCGATCGCGGTGGTTGCGCTGTTCATGGAGCCGGTCACCG10.6211180124223602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAA10.6211180124223602No Hit
CTGAGCCACCTCAGACGCTGCCGACGCACCCCCTTGTGTCGAACACGCTA10.6211180124223602No Hit
GTTACTAAGTTCTTTCTCCTAACTACATCCCATATGTCAATTTTAGCCCT10.6211180124223602No Hit
CTATCTATTCAACCGACTACGACTGTGCTTTTTTTCACACAAACTACTAG10.6211180124223602No Hit
ATTTACCCCCCTGCATGAGTGACCTCACACGTGCGCTAATGTTGTCTGGA10.6211180124223602No Hit
GTCTTATTTCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGC10.6211180124223602No Hit
GTATTTAAACTTGTTTTCCGCACTAGAACCGATTCGCGTTGGACCTATAC10.6211180124223602No Hit
CCGTTGACCAGAGCACGATCCTTTGGCATATCACCGACCTCAATGATCTT10.6211180124223602No Hit
CCCCAACAATCCTAGCCTATCCAGCCACACGCTCTGGGAACAACTAGGGC10.6211180124223602No Hit
CAGTGGTCGAAGCAGCATTAGGGATAGAGTATTACGACTCGGGAGGTGAG10.6211180124223602No Hit
GAGTCAAATTCACAAGAAGCACCATACACCCCGGCATGCAGCGGAACCAG10.6211180124223602No Hit
AGATATGATTATTTTTACCAAAACATTCTGTGGAACCGGAATGCGGTTGA10.6211180124223602No Hit
TATAACAGTTATGGTTCCCCAGACCGCCAACAGTTACTTCGATGGCACTT10.6211180124223602No Hit
CAGAACCCTATAAAACTGAACCTTATCTATAGACCAACTAGATCGAGCAC10.6211180124223602No Hit
TCGCCAGGTCATTGATGTAGTCTTTAACAAATCGGGCATGGATAGCGCAG10.6211180124223602No Hit
AGCCACCCATTATGGTCTTTCTGTTCTTCGCCACAATGGCTGGCATCACT10.6211180124223602No Hit
AGACTGAGTTTCGGAGCTCCTGGGGGAATGATAATCATCCAGGAGTTCTT10.6211180124223602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6211180124223602No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACCTGTCTC10.6211180124223602No Hit
TCAACCCTAGCCCACGACTGCGAGGAATGAACCTCAAACACCCATTAATC10.6211180124223602No Hit
CCTCAATCTTGACAACACCTCCCTCCCCCCTTCCGAGGCACAACCCCGCC10.6211180124223602No Hit
CACTTACGCTTCGTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCA10.6211180124223602No Hit
CCCCCCCCAGAATCCCCCCCCCCCCTCCCTCACTCGGCCCAAAACGACTA10.6211180124223602No Hit
AAAGGAGGAAAATTAGAGTATAATTGTGTACGAAGATAAGCCTACTAAAC10.6211180124223602No Hit
CCTTCTTCGGCTAAGTTGGTTTGGCCTCGGATTCAGCTGCCTTTTCAGCG10.6211180124223602No Hit
CTACTAGACCCTCGCCAAAAGCAAGTTCCCACGCAGTAGCAGCGGCCGTT10.6211180124223602No Hit
GCGCGGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTC10.6211180124223602No Hit
CCCAAATTCTAGCCGGCATCTTTTATGGTTAGAACTAGGACGCTATCTAC10.6211180124223602No Hit
ATACACCTATGCAGCCGCCGAGGCGACCCATCGCTTAACTCTCGGTCGCC10.6211180124223602No Hit
CATAACCTATTCACTGTAATTGCAAATAGTACACTATAAATCCTTTAACA10.6211180124223602No Hit
GGCCCAATCCGACGTTTAAAGTTTCATGTAACATTGTATCTCGACTGCGA10.6211180124223602No Hit
GGGCGAGGTTGGTTTGAAAGTAGCTTTGACATGCTCATGTGACCTGATCT10.6211180124223602No Hit
CTTCTGACGCTGCCGACGATACTACTTGCATAGCACGAGGGTGTCGGCAA10.6211180124223602No Hit
CTTTAATATACGCTATTCTAGCTGGAATTACCGCGGCTGCTGGCACCCGA10.6211180124223602No Hit
ATACACGTCTGCCGCTGGGGCCGATACTCCTTGTGTAGGTCTAGGGGGTC10.6211180124223602No Hit
GCTTTACTTCGCTGATGGCTTTGTCTTTGCATTTCATAAATCCGTAAGCT10.6211180124223602No Hit
CAAACCCGGAGATATCTCGTTCTCCCCGAAATAGCTTTAGGGCTAGCGTC10.6211180124223602No Hit
CTTATACAAATCTGACGCTGCCGACGATATAATTTGTCAACATCAAGGTT10.6211180124223602No Hit
TGAGCGGTACAGAAGGCAGCCCGCGGCGGCAGGGCATACGTGCAGTGGCA10.6211180124223602No Hit
GGTGTTAACCTCGGTGACTTCCGTTTCGGTGATGCAATGAGCAACTCCAT10.6211180124223602No Hit
AGTCTAACCTGCCCACTGAAAATTTTAAATGGCCGCAGTATTTTGACTGT10.6211180124223602No Hit
ACTTTCTGTATCACAGCGGAATATACTTGTATATTCCCCACCCATAACCA10.6211180124223602No Hit
GAAGGCATGCATCAAGACAAAATCACATGTCTGAAGGCCACAGGTATCAT10.6211180124223602No Hit
TTTTTTTTTTTTTTTTTCTGTTTACAATTCATCTTGGGTTTTTCCATGTT10.6211180124223602No Hit
AAAAAAAAAAAAAAAAAAATTATTACATCCAGCCCCGACACCCGGAGGAG10.6211180124223602No Hit
GACTTCCCACGTGCCACGACGCTCACCACTCCCGTCGTAATACTTCGGAC10.6211180124223602No Hit
TGGAAGTATCTAGGACTACCATGGTTGCAACGGGTAACTGGGAATCAGGG10.6211180124223602No Hit
GGAGTGGAGCCTGCGGCATAATTTGACACCACACGGGAAAACTTACCAGG10.6211180124223602No Hit
CAGCCCTACATGGGGCCACGCCCCAGTACAACACCTCCCCCTACTGCGTT10.6211180124223602No Hit
CTGCGCTGAGACACCACCAGCGCCCGAATAAGCACCAGCGGGCAAGTACA10.6211180124223602No Hit
CTCCGCGCAAATCTGACCCCCGACGGGGAGCCACTTATCACAACCCCGCC10.6211180124223602No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGTTCTCGGTGGTC10.6211180124223602No Hit
ACTCTAATATCCTATATTAGGGCTGGAAATTCCGCTGCGTACGGTACAAA10.6211180124223602No Hit
GAGCAGAAACACGCCACCGGGAACGGAACAACTGGCAATGGAACGGCGAT10.6211180124223602No Hit
GATTATTATTATTTATTATTTTTTCTATTACATTTATTTTATTTTGTTCT10.6211180124223602No Hit
TCGCCAGCCGTCAGCAACACACCCTGTACGGCCAGGCGGTCGTGACCCGC10.6211180124223602No Hit
TATAAACTTCTGACGCTGCCGAAGATACTCCTTGTGTAGATCTCGGTGGT10.6211180124223602No Hit
GGCGACTGAGTGCTCCACGCACCTCAGCACACCCCCCCCGTGAGGGAAGA10.6211180124223602No Hit
CTATATAGTGCACCCGGAAGCGGAATCAGCTGCGACGTCCACCAGGCTTA10.6211180124223602No Hit
GTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGG10.6211180124223602No Hit
CATCCTACTCAGCCGACGAAACTCCCTAAGTAGATCAAGGTGTTCTCAAC10.6211180124223602No Hit
ATCTACGAGTAGTTTCCGCCCCTCTCTATATTTCATACCCGATAATTTAG10.6211180124223602No Hit
CTACACATCTGACGCGGACGACGAAAGTCAATGTTTAGATTTCTCTGGTC10.6211180124223602No Hit
ATACATGCCAAAGGGCTGGACCCGAGGAATTACCACTCTGCTGCCTCAAC10.6211180124223602No Hit
CATCTCACCCTGCCGAAGAATAAAAGGCTGAAGATTTCGAGGTCTGCCGT10.6211180124223602No Hit
CGAAGCCCAGGAACGAGCGAAAGAGCCCACGACAAAACGGCGATGTCGGA10.6211180124223602No Hit
TTCGGGTAAAAGAGCACCATTTTCCACCGCTTCCACCCCAACTACATATG10.6211180124223602No Hit
ATACCCATCTGACGTTGCCGACGCCCCATCTAGTTCCAATATACGTGGTC10.6211180124223602No Hit
GGAGGATAGTTTAAATGCTTTAACAAGAACCAAATGGACGGGCCGACCGG10.6211180124223602No Hit
CGCATGACGTTTCACCCACCGCGCACATAGGCTGATTCCTACAAACAAGT10.6211180124223602No Hit
CATCTAACGCTGCCGACGATACACCTTGTGTAGATCTCGGTGGTAGATGC10.6211180124223602No Hit
CACATCTGACGCTGCCGACGATACCCCTTGTGTAGATCTCGGTTGTCGCC10.6211180124223602Illumina Single End PCR Primer 1 (95% over 21bp)
CCTCCTATCCCTCCTCGGCCGCTGTTACCCGCTCCCGCTCGTTCCTGCCG10.6211180124223602No Hit
CATCTGACGCTGCCGACGAAACTCCTCGTCTAGACCTCGGGCTACGCCCT10.6211180124223602No Hit
CTCCAACACGAAGCAGCGGTGGGAAAAGGACACAAATGGCGCATCCCTAA10.6211180124223602No Hit
GTCCCGGTGGTATCATCTGCAACATTGGATCCGTCACTGGATTCAATGCC10.6211180124223602No Hit
TCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAA10.6211180124223602No Hit
GGGGATTCGAGAAGACATGACAAGAGAGAGCGGTCCCTATAAAAAATCAA10.6211180124223602No Hit
CCGAAGGCATTTGTTTGATCTTCTTCTCTTATTCCATAAAGATAGTTCTC10.6211180124223602No Hit
CATCGCAAGTTCCCACCGCAAACCCGTAGGTCCATCCCAGTGAAGACAGT10.6211180124223602No Hit
CGCATAACCAACTCATACGACCGACGAAGACTTCGACCTCTCGCGTCCTA10.6211180124223602No Hit
TAATAGTACCGGCCCACAATAACACTCGTTTAAGAGCACTAATGCAGGTT10.6211180124223602No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC10.6211180124223602No Hit
CCGCTTACGCTTCCGTCTAAACCCCCCTTTCAAACCTGTCGGTTTACCGT10.6211180124223602No Hit
GAACGGGTAACGGGAAAGCAGGGTTCAATTCCGGAGAGGTCGCCTGAGAC10.6211180124223602No Hit
CTACTGTGAATGGGCTTATCAACTCCACCCACTGTTCGGCTTTCGTTGGT10.6211180124223602No Hit
GGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC10.6211180124223602No Hit
CATCTGACGCTGCCGACTAGACTCCTTGTGTCTTTCTCGGTGGTTGCCGT10.6211180124223602No Hit
AGGAGTGGGTGAATGTATATCTTGAGCTCCCCAGGTGGGAGACACCTAAC10.6211180124223602No Hit
GGTAACGGAAGAAGTGTAAAAGCAGAGCCAGAAGGCCCAATACCACATCC10.6211180124223602No Hit
CTCTGAAAGTGATCTAATGGGTCGACGCACGCAATCCCCGCAACATTTGG10.6211180124223602No Hit
AGACCCACCAGTCTCTGCCCACCCCTCTCCCGTCGAACCTCCCCGACCAC10.6211180124223602No Hit
CCTAGTTTCCGAGCTAGTAGTATGACAATGACCATCCTAGCGTCCTTAAA10.6211180124223602No Hit
CATCTAACCCTGCCGCCAACACCAATTGTGTACGTCCGAGGCGTCGCCTA10.6211180124223602No Hit
AAAACAAGATCGGAAGAGCCCCAGTTTAGATCGTACGCCGTATCTTATAC10.6211180124223602No Hit
ATTGAGCACGACATTGATAGGCTCTCTCAAAGACACTTGTTCCCCTTCAA10.6211180124223602No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers