FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s61.352000000d82e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s61.352000000d82e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3358790
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114890.34205770530458884No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT81920.24389735589304481No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG66660.1984643279276168No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA60430.17991598164815306No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT55630.16562512095129495No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC52880.15743764867705334No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT49430.14716609255118657No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA48770.1452010992053686No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC48160.14338496899180955No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT46760.1392168012885593No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG44610.13281568660142493No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG40860.12165095168200453No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA36900.1098609916070966No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA36370.10828304240515187No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA36230.10786622563482684No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC34410.10244760762060147No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT34280.10206056347672823No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA34130.1016139740799514No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA33660.10021466063671738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACGT59750.041.653578
GGGAGAC64800.041.0460936
AGACGTG60250.037.8392949
GGAGACG69400.037.572647
AGGGAGA35100.034.5052725
TTCCGAT129200.032.055782
CCGATCT129750.031.810084
TCCGATC130950.031.8087393
CTTCCGA155800.028.3509651
TGGGAGA31400.026.6216755
CTAGGGA5850.023.5447733
TATGCTT29750.023.4683595
CTTGTCT31750.022.4391489
CAGGGAG28400.022.4098434
ATATAAG19500.022.1645622
GATCTAG11650.022.0154066
ATGCTTG30500.021.9572586
TGCTTGT32800.021.7208217
CGGGAGA24300.021.695475
TATATGC33300.021.5369933
Produced by FastQC (version 0.11.2)