FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s6.352000000d7bc8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s6.352000000d7bc8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3638
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA872.3914238592633317No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT601.6492578339747113No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT541.4843320505772404No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT521.4293567894447499No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT130.35733919736118747Illumina RNA PCR Primer (96% over 25bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.2473886750962067No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG60.16492578339747113No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC50.13743815283122596No Hit
GCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.13743815283122596No Hit
CTTCCGATCTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10995052226498077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC300.00951516347.58
CCGATCT300.00951516347.59
CACATCT651.0835569E-436.5384644
ACATCTG550.002254221134.5454565
TACACAT600.003454608631.6666682
ACACATC752.515128E-431.6666683
ATACACA752.515128E-431.6666681
Produced by FastQC (version 0.11.2)