FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s57.352000000d8261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s57.352000000d8261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.5905511811023622No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG40.26246719160104987No Hit
AAACATAATATATTTGTTGGCCGAGGGACGGAATATCACCGATAAAAAGC40.26246719160104987No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC30.19685039370078738No Hit
GTGGCATGCTGGCCCTTGCTGGCTGAGTGGAAACTGCTAGTTCACACCCA30.19685039370078738No Hit
GCTCAAACCTCAGCATCATGCAATATACCCATATAAAAACCTACACGTGT20.13123359580052493No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTTGTC20.13123359580052493No Hit
TTGCTCGATGGTGACATGGGCGCCATCGGCGGATCTGTCGATGCCCTGTC20.13123359580052493No Hit
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT20.13123359580052493Illumina RNA PCR Primer (95% over 24bp)
GTCTTCCTTCGTAACCGTGGCGAACAGCTTCTTCTGCACATCCTCCCCAC20.13123359580052493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC155.393202E-495.03
TTATACA155.393202E-495.02
CTTATAC155.393202E-495.01
CACATCT200.001692124571.257
TACACAT200.001692124571.255
ATACACA200.001692124571.254
ACACATC250.00410112457.06
Produced by FastQC (version 0.11.2)