FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s55.352000000d822e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s55.352000000d822e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGTATCTGACTGGTAACAGAAAATAATCAGTTTATTTATATGATATC10.641025641025641No Hit
GGACTTATTGATGTTGTAGTCTTAAGTCTACTTATTGGCTACCTTATCGA10.641025641025641No Hit
CTTTAATATACGCTATTTGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10.641025641025641No Hit
CCCTTAGTTTGGTGCTGGACCATGGTGCCTGTTTTGTTCCTTTAGATATC10.641025641025641No Hit
CCTCGGCGTCTGCCCACGACTCCTTGCATTTGAAACCCGCGACCGGTCCG10.641025641025641No Hit
CTCAAGCCCTCGGATCGGGCCTTGCGGACAAGAAGGTGGCGATGGCGGGA10.641025641025641No Hit
TTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATG10.641025641025641No Hit
ATACACATCTGACAATGCCGGCGATCCTCCATATGCAGAGCCCCCACGTC10.641025641025641No Hit
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT10.641025641025641Illumina RNA PCR Primer (95% over 24bp)
GAGCTGTTAATGGCCTGCGGAAATTATTCAAACTTCGCCGTCCCACCACT10.641025641025641No Hit
ATGCAGAGTCCCGCATCCCCCCCGGCGACATGCCTCTGTTTCCGGTGAAG10.641025641025641No Hit
AACAAACGCAATAGGCGCCGCAACCCCCAGGCCCAGGGATCTGCCGGTGA10.641025641025641No Hit
GTATCTAGGACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT10.641025641025641No Hit
AACCACTTAGAGGCCCAGTCGGTTGTCAGCAGCCGCCGTAATTCCAGATC10.641025641025641No Hit
CTTATACACATCTCACGCTCCCGACCAAACGCCCCGTTTAGATCTCGCTG10.641025641025641No Hit
ATCATGGCCCCCTGCAGCCTGGTTCTCCTAGGCCTTAACCACCCTCCCAC10.641025641025641No Hit
GCTAAAGATACCAAGAAAAGATTAGTTTCTTTCCTTATATATCACTACAT10.641025641025641No Hit
CCCAGTACTCCTTCTCTCCGACACCCGGCTATCCCCGGCCCTGTACACCA10.641025641025641No Hit
GCCCCTTACTCTTGCCACGACTAGACAACAACCGTCCCCCACGTTACAAG10.641025641025641No Hit
CTTATACACATCTGACGCTGCCGACGATACCCCTTGTGTAGATCTCGGTG10.641025641025641No Hit
AAAGAATTTGGTGGCGTTAAACTGCTGTTTGAACCATGCTCCTCTTGCAT10.641025641025641No Hit
ATTCCCGATTCTGCCATCCCTGCTCCCGAGACACGACGGAGAGCAGTTTC10.641025641025641No Hit
TTACAGTTGTGGTTAGTACTATACCTTTATGTCTGTTAGAGTATTTCCGG10.641025641025641No Hit
CCTACCTTCCTAGGTGTTACCGCCGAGCGGAGTCGCCTTACCACGATGGG10.641025641025641No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC10.641025641025641No Hit
ATCTTGTGGTTCGCAGAACCCTATGAACTAATGTTATGTTTTTTTTTTGG10.641025641025641No Hit
CATCTGACGCTGCCGACGATACACCCTGTGTAGATCTCGGTGGTCGCCCT10.641025641025641Illumina RNA PCR Primer (95% over 23bp)
CCACGGGCAAACGGGGCGAAAGCACGAAAAAATGTTTACCGCCAAGATTC10.641025641025641No Hit
CATCTGTCTCAGACGTTTATAAAGATAGAAACAAACCTGGGTTACACGGG10.641025641025641No Hit
ATAGGCTACCACTCGGGAATTACTTGCGATCATAGTGCCGCATGTAGGTT10.641025641025641No Hit
AAAAAAAAAAAAAAAAAAAAAAACAACTACCCCCCAACCACCCACAGGAG10.641025641025641No Hit
ACACAGTACAACGGGACAAAAAAAAAAAAAGGAGAAGAATCTACACACAC10.641025641025641No Hit
GCACGATCCCCCCCATACCACGGTCGCCAAACGCCGTCGCCGGACCTCAC10.641025641025641No Hit
CTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGA10.641025641025641No Hit
GTTCTGCTTTCATTGGTTTTCAGATCCAGCGGTACTGATTCGTAGACGCC10.641025641025641No Hit
GTACTACGCTCGAGCGCCATCCATTTTCAGGGCTAATTCTGTCGCTTATA10.641025641025641No Hit
ATCCCCCTCCGAACCGGGAGACAAAAAAAACCGCGCCAATCTCCGTGCAC10.641025641025641No Hit
CATCTAACCCTGCCAACTACACCCCCACCCTAACCCACCCCACTCCCCCT10.641025641025641No Hit
GTGTGCGTGTGGGTGGTGGTGTGGCGACCAGGCCGTGGTCGTGGGGGGTT10.641025641025641No Hit
ATACACATCTGCCGCTTCCTACTATACTCCATTTGTGCACCCCCATGTGC10.641025641025641No Hit
CCTTTAATAGCAGCCAATCTAGATCCTCAAGAACCCTTTGAATTCCGACC10.641025641025641No Hit
AAGATAGATGATATGGAGAACGTGACACGTAGCGTCTTTGAGATACAACA10.641025641025641No Hit
CTGTTTGGCATTCGTAAGATGTGGGACTCAAAGGCTGTTAACGATCTAAC10.641025641025641No Hit
CATTTTTATGTTTACGGCGAAGACTGGCAGCCAGGATCTGAGACTCGCGT10.641025641025641No Hit
GTATCAAGGGTTGGCCACGTTCCTCACTAAGTGGGGCTATTTCCGCCATC10.641025641025641No Hit
GCCTCACTCTCATCTCCCCACCTTAGTCTCCCAGCTTTGAACTCTCCATA10.641025641025641No Hit
GTCTAGCACCAGATCTGTGCATCTCTTCAACGAGGAGGTGCCGCGCTCGA10.641025641025641No Hit
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGACGT10.641025641025641Illumina Single End PCR Primer 1 (95% over 23bp)
ATACACATCTGACGCTGCCGACGCCACTCCCTCCCCACCTCCCCACTGCC10.641025641025641No Hit
CCCTGTAGCAGTGTGAGCAGATCCTGTCCTGGCGCTGCCTCATTGATCTC10.641025641025641No Hit
ACACCCCACTGACTATTACCACGATGCGCCCTGCTGACTTCCCGGTGGTT10.641025641025641No Hit
CTTATACACATCTGACGCTGCCGACGAAACCCCGTGTGTCGATCTCGGTG10.641025641025641No Hit
TTCGTTTTTGCAACTCTCCTCCAGTCCAAATTCGTGAGTAGACCCAGCGA10.641025641025641No Hit
GGCTGGTGCAAGGGACGCATGAATGGGCGCGTCGGACTGTATCCGGCCAA10.641025641025641No Hit
CATGTTCTTATATTAAGCAAAAACCAAGCGAACGCCATATCTCTATATCA10.641025641025641No Hit
ATACAAGTATTTAATCACATATAAGAACTCCACCGGTAATACGCTTACAT10.641025641025641No Hit
CCCCGATGTTGGCCATTGATGGCGTCGATGCTCAAGGAACTCAGTTTGGG10.641025641025641No Hit
GAATTTATCATTGCATTCGCTTAGATCCCATCTCGACTGTCAGAGAGCCT10.641025641025641No Hit
CTCTTCCGGATTAAGTTTCAGCTTTTCCCCCCTCCTTCCCCCCGAGCCCC10.641025641025641No Hit
GTACAACGGCCTCTTCCAATCCCCAACTTACTACGACTGTTAACCCATAA10.641025641025641No Hit
GTTTTTCTCTAAAGCCAAATTGATGTGTTGGAAGTGTGTTGTTTATTCTA10.641025641025641No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG10.641025641025641No Hit
AGACCCATCTTACGCTACCGCCGATCCTCATTGGGTATATCTCGGCTTTG10.641025641025641No Hit
CTTATACACATCTGCCGCAGCCGACGATACCCCTTGTGTAGATACCGGTT10.641025641025641No Hit
GTCTAAGTACATACGAATCAAAAGTGAAACCGCAAAACGCACATTCTATC10.641025641025641No Hit
GTGTTTCACACGGAGGTTGCGTAGCTCGCTAAACTTGGCCAGCTCATCGA10.641025641025641No Hit
GCAAAATGCGATAACTTGGCAGTAGAAGTTAATAAAAACTCACATCAGAA10.641025641025641No Hit
GTACGTTCTTGTACCGACCCCCGACCTTTCCCCTGTGTGCCCCCCCAACT10.641025641025641No Hit
TCCCTACTCGCATCGCCTCCCTTCTCTCCCCTCTGTTCCCTTATCATCTC10.641025641025641No Hit
CTTTTGATGGTAGTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACA10.641025641025641No Hit
GTCGGTGATCAATCCGCAGAATCTGCAATTTCTGTCGTTTTCTCTTGCCT10.641025641025641No Hit
GCCCGGATCTTCGTGATGTTGCTCAAACTATCCAGTTCGCGGTCCCGTTC10.641025641025641No Hit
GTTCAAAGTAATAGTACCGGCCCACAATAACACTCGTTTAAGAGCACTAA10.641025641025641No Hit
CTGCCCACCTCCAGTTGTGTTGGAAGTGATTGCCTAATATCTCTCAGGCC10.641025641025641No Hit
AGAGCAGAGAGAAGAGAAGAGAGAAGCGACAAGAGAGAAGAGAAGAGAAG10.641025641025641No Hit
AAAAAAAAAAAAAAAAAAAAAAAAACAAAAACACACAATAAAATAACAAA10.641025641025641No Hit
TGATAACAGTGACCACTCGGGAACCGCTAGCCATTAATCCAACCTACAGC10.641025641025641No Hit
CTACACATCTGACTCTGCCGACGCCCCTCCTTGTGTATATCTCGGCGGGC10.641025641025641No Hit
AGACCCATCTTCCGCTGCTGACGATACCCCCTGTGTCAAACCCGAGAATC10.641025641025641No Hit
CATTTTATTTTACTTCAGACGGAACAGAAACAAAATTTGCGTATATATGT10.641025641025641No Hit
AAAAAAAAAAAAAAAAAAAAAAAAACAAACACCCTCCCCACACACCCAAA10.641025641025641No Hit
AAAAAAAAAAAAAAAAAACAAAAAAACAAATATTATCCAAATTGCATCCA10.641025641025641No Hit
GAACGAATTTGAGAGGGAGGCGAGCGCTAAAAGCGTATTTGTAAAGTTCT10.641025641025641No Hit
GTTTATATGTGTTTTGGTGGGAAAAAGATTTTGTGACGCTGCCGACGATA10.641025641025641No Hit
GGTAAATGGCAATGGCGCGATTTCTTTTTGTTTGCGTTTGAGCAAGTCAC10.641025641025641No Hit
GTCAATCAGATCAACCGCCGCAGACGATATCACTAGTGTACGATGAAGTG10.641025641025641No Hit
CACGTCTCCCTTGTTATATCGCAATACCACACCGCTCCCTGGGTCGAGCG10.641025641025641No Hit
CCCATAAACATATGCCTATTCCGACGAAACACCGACTGAAGACGCCGGCA10.641025641025641No Hit
TATACACACCGGACGCTTCCGAAGATACTCCGTGCTTCCATCCTCCTATT10.641025641025641No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.641025641025641No Hit
ACCAACTACCATTTCTAACTATCCCTATTACTTTTACCAGGATTTAGCGC10.641025641025641No Hit
CTGTAAGCTTAGCCAGGGGGCAATTAAAATTCAAGCGAGTACTCATATGG10.641025641025641No Hit
CTCTGAAGGTAAAAAGTACTTTTTCCGTTGAATGCAATTCTTCAAATGTG10.641025641025641No Hit
GATATGATGATGCCGACGATATTTCTTGTGTAGGTCTCGGTGGCTGACGC10.641025641025641No Hit
TTCTTGCCCTTTGGACTTTTACGTCTCCGATAGAGCACCAAAACTTAAAT10.641025641025641No Hit
GAATGGGAAAGGCCTTGGTCGCTGGCAGGAGGGAGGGAAATGCCAAAGTC10.641025641025641No Hit
CATCTCACGCTCCCGAGCAAACACCTTGTTGATCTCTAGGTAGTCCCCGT10.641025641025641No Hit
GCCCTCAATCGAGCAGTCATCGAGCTGAGGGATCTCGGCAAAGTGCATAA10.641025641025641No Hit
ATGCCGTACAAGGCACCCGCCTCAGTTTTACTACCCACCTGATCCTTTCC10.641025641025641No Hit
TAGAGGGAACGGTCAATCTGGAGAAACCCGTGGTGGAGTGGGGGTACGTC10.641025641025641No Hit
CGTTAACAAGGACCAATTGGAGTGCCATTCTGGTCCCCGCAGCCGCGGTA10.641025641025641No Hit
GCATAGCACACGTCTCCCCTTAGAGATCGGAAGAGCACACGTCTCCCCTT10.641025641025641Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCGAAGAGGTCACCGAGCTGCTGGCCAAGGACGAGTGCCTGTCTCTTATA10.641025641025641No Hit
TAGAAGCAGTTTATGCGTTAGGGATTTTGTCACCGGTATACACGTACTTC10.641025641025641No Hit
CGCTGGGGGAGGCGGTGGCCACCCCCCCGCGCCGTGCCCAAAGCAGTCCC10.641025641025641No Hit
CAGCCTAGTTGTCTTGGCAACGGCCCACCGGTTAGGACCTCAAATATGGA10.641025641025641No Hit
CGTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG10.641025641025641No Hit
ATTAGTGGCACAGCTTAATGCTCAGACACACCCAGCTGCATGGCCAAGGA10.641025641025641No Hit
CTTTACACCTCTTACTCCCCCGAAGAGACCCCGTACGTCGATCTCCGTGG10.641025641025641No Hit
AAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAATAAAACAGACCAAGA10.641025641025641No Hit
GTCATACCTCACTGAGCTCGTAATAAAATTTCCAATCAAACTGTGTTCAA10.641025641025641No Hit
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTGGCCGC10.641025641025641Illumina Single End PCR Primer 1 (95% over 22bp)
GGCGGTATCTTAGCACCTTCCAACCTCTAACGAACGTTCCTGTGTATTGA10.641025641025641No Hit
TTCAGGATTTGTCTGCCTTAACTTTTATCTGATCAGTCTTACCATAAACA10.641025641025641No Hit
CTCAAATGCAAACCAAAAAAAAATAAAAGAAAACTGCAAAAAGAAATAGA10.641025641025641No Hit
TTACAGCATTTCCTGTATCTATTGAATCTCCTACAGCTTCATAGTCTAGG10.641025641025641No Hit
GCCATAAAAGTAGCTACACCCAATTGCTAGCTGGCATCGTTTATGGTTAG10.641025641025641No Hit
GCTTATAACAAAATTAATGATCTCATCCTTTTGAAGCGTGCTCAACACTT10.641025641025641No Hit
GCATTATTTGGAGTAATTGCAGTGCCCCTCGTCACTGACCATATTACGCT10.641025641025641No Hit
AGTATCTCCGTTATTGTTCCTTAGCCCGTCACCCGTTACTCGGATACCTG10.641025641025641No Hit
CTCTTCAGATGCTATGGGGTAAGCGTAAAGGGGGATCAGGTGCCGCGCCC10.641025641025641No Hit
ATGCACATGCCTCCAGCCCCTGGCCACTACGCGCTCCCCCCCAACAGCCG10.641025641025641No Hit
TTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTCCTAGATCTGTCTC10.641025641025641No Hit
GTGCTCTTCCGAGCTTTTCTTTTTCTTTCTTGTTTTTTTTTTTTCTATAA10.641025641025641No Hit
GTATTTAATCACATATAAGAACTCCACCGGTAATACGCTTACATACAGAA10.641025641025641No Hit
TTCCCCCGGGTATGGCTCCTCCCCATTTCCAGAGGTCCGTGAGCCGGGAC10.641025641025641No Hit
GGACAAATAGAGATAGACAGGCCCATACGAAGAAATATGCAATTGAATGC10.641025641025641No Hit
GTGCTGTGAGGAGTTCAGATACGAGCTGCCGGTGCTATTGCCCGCCTGCA10.641025641025641No Hit
TCACCTCACAGGGGCCATATAGACAAACTGACTCAGAGCTGAACTGTTTG10.641025641025641No Hit
CTTAAACACATCTCACGCCGCCGACGATATCCCACGTGTAGCTCTCGTTG10.641025641025641No Hit
GCCCGGATACACGGACGGTGGTGCCCAGCTTGGTGTGGCAGACTGTCAGG10.641025641025641No Hit
CAGCTGGGAGTATGATTTTACAGCTCAGCGAGTAATCATGTTGGACTTCT10.641025641025641No Hit
AAAAAAAAAAAAAACAAAAAAAAAAAAAAAACAGCCTTCACCCTCACAAA10.641025641025641No Hit
CAGAAGCACACCCGTGCGCTTCCCCTCTCTCCTCCACACACTTGACCCCC10.641025641025641No Hit
CTTATACACCTCTGACGCTGCCCCCTATACTCCCTTTGAAATTCTCGGTG10.641025641025641No Hit
ATACACATCTGACGCTGCCGACGCTACCCCTTGTTTAGATCTCGGACGTT10.641025641025641No Hit
ATACACATCTGACGCTGCCGACGATACCCCTTGTGTAGATCTCGGTGGTC10.641025641025641No Hit
GAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAA10.641025641025641No Hit
GGGGCCGCTGGCCTCGACGTGTCATCACGAAGAAGCCCGAAACCAGACAA10.641025641025641No Hit
CATCTGACGCTGCCGACGATACGCCTTGTGTAGAACTCGGGGGGCGCCGT10.641025641025641No Hit
TACATAACCCAATGAACGTTAGCAACTGACACCAACTCAATCGGCGCGCT10.641025641025641No Hit
CGCCTTACGCTGCCGACTATACCCCTTGTATACATCTCGGTGGTCTGCGT10.641025641025641No Hit
CTTATACACATCTGACTCTGCCGACGATACTCCTTGTGTAGATCTCCGTC10.641025641025641No Hit
CCCATATATATTCATAGTTCCTAATTCGTTTAATTCTCAAAAGTTGCCGC10.641025641025641No Hit
ATACCATCCGATTAGGATTATTATTATTCTTCTGCAACGCAGTGCTTTAG10.641025641025641No Hit
GCATCCCAGCGTATCCGTCCACTTTCGAGTTTGCAGCTTGCTATGTTATT10.641025641025641No Hit
AAACAAATATAACACTTCCGACGATACCCCCTGTCTACATCCCGGTAGTC10.641025641025641No Hit
ACTCGTAATTGATTGTCTCCGCCATTTTTTATTAGCTTTTTCACGCAGTA10.641025641025641No Hit
AACCGGTGCTCGTAACCTTACAGCACCGGGCAGGCGGCAGTCCGTCTACC10.641025641025641No Hit
GACTGAGATGGTTGTTGGAAGAGTAGAAGAGCACCCAACAACGGAGCACA10.641025641025641No Hit
CTGTGACATTTTCACATCCCGTTTGTGGCCAGATTTTCCCTTTGTTCGTC10.641025641025641No Hit
TCCGTAAACAACTAATTTTAGCAGACCACACACGCTTCGGACGCCAACTT10.641025641025641No Hit
CCGTGTCACGGCACAGACGCCCCCCGACCCGGCTCGGTGAAGGACCCGCT10.641025641025641No Hit
GAAGCAAAGAGAAGTGAATAGAAGAGAAGAAAAGAGAAGAGCCGCGGTCA10.641025641025641No Hit
CAACAGGATCTGCTGCACAAATTTGTGCCAAATGTACAGACACAGTGTAA10.641025641025641No Hit
TGGCTCACGAAACGGTTATCGAAGAACGGGACGGCATCGCTGTCCGCCTG10.641025641025641No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers