FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s53.352000000d81f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s53.352000000d81f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4017912
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT234250.5830142621341632No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA204830.5097921507489462No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT164060.4083215361610707No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA138920.34575172378090907No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT60400.15032683642648223No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT44700.11125181437522773No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT43380.1079665258970331No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41270.10271504204173709No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA41030.10211771686388355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT91750.037.269222
TCCGATC93600.037.090733
CCGATCT92300.037.047144
GAGACGT34400.035.7595638
GGGAGAC39150.033.6037756
CTTCCGA106650.033.4428251
AGACGTG36300.030.7476629
GGAGACG44050.029.111787
AGGGAGA27900.021.618395
GTCCTAG26150.021.430591
TAGGACT35450.020.6313824
CGATCTC36650.019.9558685
CGATCTG74500.019.8256765
TCTAGGA37850.019.8250852
AGGACTA38650.019.4147345
CTAGGAC37500.019.1235963
TGGGAGA23850.019.1164265
TATGCTT28600.018.9305255
ATCTAGG38400.018.5517061
ATATAAG21100.018.456752
Produced by FastQC (version 0.11.2)