FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s5.352000000d7baf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s5.352000000d7baf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3447694
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT58730.17034574414086634No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49790.1444153686493059No Hit
TCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCT46870.13594593951783424No Hit
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGT46460.13475673885211392No Hit
ATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGC42700.12385089860062987No Hit
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTA40460.11735380228059683No Hit
GTTATGGCTAGGGGGTCATTGCGACTTACCAAACCATTGCAAACTCCGAA39610.11488838626629858No Hit
GTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCC38950.11297406324343169No Hit
TGGTAGGAGAGCGTTCTGTAAGCCTGCGAAGGTGTCTTGTAAAGGATGCT38840.11265500940628721No Hit
CTACTAGGGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTC38800.11253898982914376No Hit
GGTTAATATTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGG37650.10920342698626966No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT37160.10778218716626244No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC35430.10276434045480834No Hit
GTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTGGCTGTTG34860.10111106148051423No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA34790.10090802722051317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT68200.050.2080464
TTCTATA8600.036.4475142
CGATCTC21450.036.311065
TCCGATC95850.035.57583
GATCTAG10600.034.9491276
CGATCTG40150.033.9483575
TTCCGAT107950.032.336062
CTTCCGA116400.030.8046571
GAGACGT15750.030.7586448
ATATCAT10800.029.4644726
CGATCTA28250.028.5793845
GGAGACG18650.028.0131177
AGTATTG7250.026.8592248
TGCTCTT37700.026.7064952
CGATCTT19850.026.5573585
TATAGAA7950.026.2849982
TATCATG12300.026.2573817
TCTATAT12300.026.255863
GCTCTTC39050.025.6616063
ATCTAGC7850.024.8062887
Produced by FastQC (version 0.11.2)