FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s49.352000000d817f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s49.352000000d817f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC40.28632784538296346No Hit
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT40.28632784538296346Illumina RNA PCR Primer (95% over 24bp)
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA30.21474588403722264No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG30.21474588403722264No Hit
CTTATACACATCTGACGCTGCCGACGATACCCCTTGTGTAGATCTCGGTG20.14316392269148173No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA20.14316392269148173No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT20.14316392269148173No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14316392269148173No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)