FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s48.352000000d8156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s48.352000000d8156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTCCGATCTGCGCCGGGAATCGACGAACATTGCGCCCGGGCGCGCGC10.8849557522123894No Hit
CTCATTTTACATATCGGCAATCTCTTTCTGATTGTCCAGTTGCATTTTAG10.8849557522123894No Hit
GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCCC10.8849557522123894No Hit
AATGGTAGGAGAGCGTTCTAAGTGCGGCGAAGCATGATCGTAAGGACATG10.8849557522123894No Hit
AACTACGACTTTTTCAGTGGCTTGGTTCCGATTAGATATGTTTTACAGAC10.8849557522123894No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA10.8849557522123894No Hit
GTGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGT10.8849557522123894No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA10.8849557522123894No Hit
TTGTAGTACAGATTCCGATCGTAATTGATCACAAGGGCATTTCCCACCGG10.8849557522123894No Hit
TCACGGGGGAGTGTTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8849557522123894No Hit
TCTTCATTTCCATGCGGTGCACTTTATGCGGACACTTCCTACAGGTAGCG10.8849557522123894No Hit
GTTAAGACTACAACGGTATCTGATCGTTTTCGAACAACTAACCTTCGTTC10.8849557522123894No Hit
CATTTACAAATATCACGAATTGCGTCCTTCAAAGAAATCTTGCCTTACTT10.8849557522123894No Hit
CACCCGGGCGACGTGTGCTCTTCCGATCTCACCCGGGAGAGATTTGAGCT10.8849557522123894Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGGCTGATTCTGATTCTGAACAGCTTCTTGGGAAGTAGCGCCAGCTTGGT10.8849557522123894No Hit
ATGACAAGTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTACCTCCAAA10.8849557522123894No Hit
TTCTCCGAACCCCCCGTCCGTTAAACTCCCCGTCATCCCTTTCTCTTATA10.8849557522123894No Hit
CTAAATTATCGGTGCTACAATCATTGGCTGCTGCGTTGTTACAAACGTTC10.8849557522123894No Hit
GTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACG10.8849557522123894No Hit
GCAATACACTTGGTCATCTCATGCCTGATAATCTCGCCGGGCTGTGTGGA10.8849557522123894No Hit
CCTCAGTCGCTGCCGACGAAGAGCACCGGCGTACCCTATGTCTCGGCCAC10.8849557522123894No Hit
CCCAAGGATCCATCACGGTTGTTCTCCAGCATTAGTGAAACCCTCAGTGG10.8849557522123894No Hit
CTAATGTTATGTTTTTTTTTTGGGATTATCACTTCTTAGATGCCGGAGTC10.8849557522123894No Hit
ACCCATGTCCAACTGCTGTTACCATGGAACCTTTCTCCACTTCAGTCTTC10.8849557522123894No Hit
CGGGCAATAATGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCT10.8849557522123894No Hit
CCATTATTGTAATATTTACTTCTTTAGGCTAATCCGATTTCTGTCTCTTA10.8849557522123894No Hit
AGCGGGGATCGTTGGTATCCTCGGTTTCGTGGATGGACACTTGGTATTTG10.8849557522123894No Hit
GGGCGGACCATCTCCTAAGGCTAAATACTCTCTCGTGACCGATAGTGAAC10.8849557522123894No Hit
CTTCCGATCTTTCCCGGGGCCCTTGCCAGCGTCTCCAAGTTCATTTGCGT10.8849557522123894No Hit
CTATGTGGCCTTCTACGGAGTCCTAGCCGCACTCGTTGCCATCTGCATGT10.8849557522123894No Hit
GAGTGAAATCGGATTAGCCTAAAGAAGTTAAATATTACAATGATGGCTGT10.8849557522123894No Hit
ATATTTTTCATGGTATTGATAAAGCTGTTGCCGATACTTGGAACAATTTC10.8849557522123894No Hit
TCAGTAAGAACGTCAGTGTTTCCTGCACGTACACGCAAGGTAAACGCGAA10.8849557522123894No Hit
CATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTT10.8849557522123894No Hit
CTTCCGATCTCTCGCGGGACTTCGCTATCGGTCTCTCGCCAATATTTAGC10.8849557522123894No Hit
GAACGGTGCTGGTGCCCACACCAATGTGTCGACGAAGGCAATGCGCGAAG10.8849557522123894No Hit
TTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCC10.8849557522123894No Hit
TACTCGTACACCACGTAGCTGATGGACACCGCCGGCAGGACCTTGAGGAA10.8849557522123894No Hit
ATCTTCATCTCCTCCAACATGCGATAGACCTTGACGAACTTCTTATCCAC10.8849557522123894No Hit
CAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTA10.8849557522123894No Hit
TTCCAGAGGTAGGCTCGTCCGCTCTGGGTAAGTCGGGACCTAAGCCGAGG10.8849557522123894No Hit
CAAACATCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACA10.8849557522123894No Hit
GTACAAGACCTTCGACTATCCCGACGATGACATCACCCGCAACTACATCC10.8849557522123894No Hit
CGGTTAGGAAGGACCGCATCTATATGGACAATCAGCAAATGAATGAGGAT10.8849557522123894No Hit
CTGGAATACCCACAACCCATATATACGAGTGGAAATATGAGAACTCTCAA10.8849557522123894No Hit
CATCTGACGCTTACGACGAATATGAGGGTATAGATTTTGGTAGCCGTCGT10.8849557522123894No Hit
TCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTG10.8849557522123894No Hit
ATTGCTGAGGGTCAGTGGTACCGTTATGCGCCTTCGTATGTTTCTCCTGC10.8849557522123894No Hit
CCTGTGAGCATGCTGGAGATCCGCGGTGATTACATCTAGGGGATAGTAAA10.8849557522123894No Hit
ATGTTAAAATGGATTTTTTTTTCAATGTAAAAAGTTTAGATTCTGCACTA10.8849557522123894No Hit
AACAGCCAGTTGCCAAAGATCCTGTTGTCTACTGCATTTTACCCTATTAC10.8849557522123894No Hit
TTATATTGACCATGCCGCTTTTCTTGGCACGATTAACCCTGATACCAATA10.8849557522123894No Hit
TTGGAAGACTGAGAAGTATGTTATTATTAATAAAATGAGTGAAGATTTAT10.8849557522123894No Hit
AAACGCACATAACACAGGAAGGATGCGCAGGAAACAATGGCGTATTCAAA10.8849557522123894No Hit
CTTCAGAAACGGCCTGAAAATGGCATCACATAACTTATATATCTGTCTCT10.8849557522123894No Hit
CATAAATTGTCACCAGAGTAAACTTGTAAGTAAAACTTCCTGTCTCTTAT10.8849557522123894No Hit
GTATCCTACAAAGTCCAGCGTACCTTAAACGCCAGCCTCAACTCAGCTAC10.8849557522123894No Hit
ATCCACGGTCTTCATTCAACCATCCACCCAGTGGTTCAACCTTTTACATA10.8849557522123894No Hit
ATTTAAAAACCTGCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGT10.8849557522123894No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC10.8849557522123894No Hit
AAGATAGAGTGTGAGGTTATAATGCCGAAGCGGTAAAAATTTAAACTTTT10.8849557522123894No Hit
GGCAGGGGGCCTTGTGCCGGCGATGCTTCATTCAAATATCTGCCCTATCA10.8849557522123894No Hit
GTGTAATTTTTTTTGCAAATCCTTTTGTATTTTGTATGTGTCAACTTAAA10.8849557522123894No Hit
GACGCGACGCCGTTCAACCAGATATTGAAGCAGAACGCAAAAAGAGAGAT10.8849557522123894No Hit
CATCTGATTCTGATGACGAAAAGAATAGTGTTGATAAAGCAGGAAGCACT10.8849557522123894No Hit
GACTTTTACCTTGAGAAAATTAGAGTGTTCAAATCAGGCCTATGCCCGAA10.8849557522123894No Hit
GGTTATCCCCGCCCTCAACGGTAAGCTCACCGGAATGGCATTCCGTGTGC10.8849557522123894No Hit
AATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGA10.8849557522123894No Hit
AGTTAGTCTTGCGTCAATCCAAGAATTTCACCTCTAGCAACGCAATACTA10.8849557522123894No Hit
CTTAGAAGTGAGAATGCCGGTGTGAGTAGCGAAAGACGGGTGAGAATCCC10.8849557522123894No Hit
GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCTTTACTTGTCATGCG10.8849557522123894No Hit
TATCAGAAAAAAAGTTTGAATTATGGCGAGAAATAAAAGTCTGAAACATG10.8849557522123894No Hit
GCCTAACGGTGTGTCTCTGGCTGGGCCTTACCTCTTGGTGATCCGGCATG10.8849557522123894No Hit
AGTTCTAACCATAAACGATGCCAGCTAGCAATTGGGTGTAGCTACTTTTA10.8849557522123894No Hit
TCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATGTTGA10.8849557522123894No Hit
ATAGTCTGGAGAAGCGTTTTCCGCGTCGGCTGTGTACAAGTTTCCTGGAA10.8849557522123894No Hit
ACTCTCTCCCCAACATTCCAGCCTCAGCCTCAGGCAAGCTTGGGCCAGCA10.8849557522123894No Hit
CACTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCT10.8849557522123894No Hit
GGTTAGCAATACGGTCCAGATCACGCTGTCCAATGGAGCTCAGTTTGTGA10.8849557522123894No Hit
AAAGAGGGCTTTCCTTTCGTGAAGGCCAAGCCGGAAGTAGACCTGGCCCA10.8849557522123894No Hit
CTTCCGATCTGACCGGGGAGTTTCAGCTTTGCAACCATACTTCCCCCGGA10.8849557522123894No Hit
TCTGTATGGTTTACATCAATGGAAATTAGAGTGGACCATCGCTTTTGTTA10.8849557522123894No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG10.8849557522123894No Hit
CGCCAATATTTAACTTTAGATGGAATTCACCACCCATTTTGAGCTGCATT10.8849557522123894No Hit
CCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCGGTGGCATT10.8849557522123894No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA10.8849557522123894No Hit
TTTTTAATCAGAGCTTTGCACTTTCCATGCCATTTACAAATCTGTCTCTT10.8849557522123894No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT10.8849557522123894No Hit
CTGATGCAGAGCGTGAAGGAGACGGTGCGCGCCGCCGAAGGAGCCAGCAT10.8849557522123894No Hit
GTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAG10.8849557522123894No Hit
CGAGAGAAACTCTTATCAGGGACACTTTCAAACGCGCGATGGCGTCTTGA10.8849557522123894No Hit
CTCTTTTATGGGGGCACTAATCCCATTCCTGAGGACTCCACCGTCATGAC10.8849557522123894No Hit
TTTATTAACATCTGACGCTGTCTACGAAGAGCATAGTGTAGATTATGGAG10.8849557522123894No Hit
GATATAGGTCGAAACAAAATTCATGATGTAACTAAAGCCCCTTTCCTTAT10.8849557522123894No Hit
TGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAG10.8849557522123894No Hit
TTATTGTAATTGTATTTTTAATTGCAATTGTACGTGTCTTGTTGTTGATG10.8849557522123894No Hit
CCATACGTACCATAGAAATCCGGAGGGAGGCCAGTCGTCGCCCCAGGGAA10.8849557522123894No Hit
GGTGATTTCTGTGTGCAGACACAGTGGATGATGGGGCTAAATTTGATTTG10.8849557522123894No Hit
GATTAGAGGTCCTCATGTGGTCAGAGAAATGGTGAGTGGTCTGATAATGG10.8849557522123894No Hit
TTTTTTAAGAGTGAGTGTGTGTTGTGTGAAGCGCGCCCGCGGGTGTGTGG10.8849557522123894No Hit
CCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTT10.8849557522123894No Hit
ATTATAAAGTGTACGAGGTTTAAAATTATTAAACAAACCCCCCCAGTAAT10.8849557522123894No Hit
ATCGGCATCACATTAATATCTACACGAACATGCAGACCTGCAATAAATAT10.8849557522123894No Hit
TCCTCGGGGAGCTCTTCCTCGATGGCGGCCGCATCCTCGTCCGGCTCGTT10.8849557522123894No Hit
TTCCAGTTCCTCAAGGCGCTGGGAAACAATCTCATCGGTCACCTGGAGCT10.8849557522123894No Hit
GGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCA10.8849557522123894No Hit
TCTTGGGCCTCTCCGGCGGCCGCGAGTAGTACTGTTGGAATCCATCTATG10.8849557522123894No Hit
GTCTATTGGTTCCAGATCGTGTCGGACGCGTTCTGCGACGATTTGGTGGC10.8849557522123894No Hit
ACCGCAACGCACGCGAGTGGAGATCACGTACTCGTTGGTGGGGTCAACGT10.8849557522123894No Hit
ATCACATACGGCCATCGGCCCATGTACTGCAGCAATGACAACGGAGATGA10.8849557522123894No Hit
CGTATTCACTGATGACGCTGCTGGCGTGGTGGATAGTTTACATGGTATTG10.8849557522123894No Hit
ACATGCACATCCTAATATTCAAGAGGCAGTTCGCTCGACATATCTTACGC10.8849557522123894No Hit
ACGAAAATAGACACGCAAAGCATTGGGATTATCATAAAACGCCTCTAATC10.8849557522123894No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)