FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s47.352000000d8112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s47.352000000d8112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3277084
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT279240.8520990002087222No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA241610.7372713058316479No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT203330.6204601407836967No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA178140.5435930235538667No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58580.1787564798461071No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT55290.16871706675813009No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT51850.1582199296691815No Hit
CTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTC49710.15168973392198673No Hit
CTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTC48410.1477227925802329No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT46390.14155877603381542No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAG45510.13887346189478206No Hit
AGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAA43290.13209914668040246No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT36100.11015890956716397No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG34230.10445261702171808No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA33930.1035371690197749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT60250.037.1257864
TCCGATC60900.036.495033
TTCCGAT61550.035.5689772
GGGAGAC26700.033.0856746
GAGACGT24700.032.688148
CTTCCGA71200.032.3489571
AGACGTG26300.027.6295599
GGAGACG32200.026.6969097
TAGGACT28250.019.500754
GTCCTAG25650.019.440191
AGGGAGA20800.019.4074025
TCTAGGG4001.8189894E-1218.9958422
TATGCTT22800.018.9547625
CTAGGAC29050.018.7999573
AGGACTA29400.018.7379675
CCTAGAT25800.018.5911753
ATGCTTG21900.018.4337256
CTAGGGG3105.6061253E-918.3833523
TCCTAGA27950.018.0103682
GACTACC30200.017.9281797
Produced by FastQC (version 0.11.2)