FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s46.352000000d80ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s46.352000000d80ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA282.206461780929866No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211.6548463356973995No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131.024428684003152No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131.024428684003152No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG40.31520882584712373No Hit
CTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.31520882584712373No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2364066193853428No Hit
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT30.2364066193853428Illumina Single End PCR Primer 1 (96% over 26bp)
CATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTGGTCGCCGT30.2364066193853428Illumina Single End PCR Primer 1 (100% over 23bp)
CACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCC20.15760441292356187Illumina Single End PCR Primer 1 (95% over 24bp)
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15760441292356187No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15760441292356187No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)