FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s45.352000000d80c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s45.352000000d80c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.39781203381402286No Hit
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT60.29835902536051717Illumina Single End PCR Primer 1 (96% over 26bp)
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG50.24863252113376427No Hit
CATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTGGTCGCCGT40.19890601690701143Illumina Single End PCR Primer 1 (100% over 23bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACG300.00889295547.58
Produced by FastQC (version 0.11.2)