FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s43.352000000d808d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s43.352000000d808d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2517
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.5562177195073501No Hit
CATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTGGTCGCCGT90.3575685339690107Illumina Single End PCR Primer 1 (100% over 23bp)
ATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTGGTC70.27810885975367505No Hit
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT50.1986491855383393Illumina Single End PCR Primer 1 (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.11918951132300357No Hit
CTTATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTG30.11918951132300357No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTGA500.001326646438.0000041
GACGCTG500.001326646438.0000046
CTCGGTG250.001466765438.00000436-37
ATCTGAC500.001326646438.0000042
TGACGCT550.002119784734.5454565
ATCTCGG300.003589556631.66666834-35
GCCGACG457.7581895E-426.38888712-13
CCGACGA557.759555E-525.90909212-13
CGACGAA500.001435326623.75000214-15
Produced by FastQC (version 0.11.2)