FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s42.352000000d8063.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s42.352000000d8063.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4883377
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160580.32882982411556594No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT84880.1738141454161741No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT59090.12100233096891762No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT54690.11199217262971915No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG54230.11105020153062113No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA52480.10746661582753082No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC51540.1055417183641566No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC51360.10517312097755302No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA49080.10050422074724111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT144250.040.9230844
TTCCGAT150400.039.8812332
TCCGATC153800.038.968723
GGGAGAC60900.036.5759246
CTTCCGA188500.034.314591
GAGACGT58050.033.3000838
GGAGACG70100.030.4889987
AGACGTG63550.027.727839
CGATCTC54900.026.2109875
AGGGAGA35200.026.0392065
GATCTAG14700.025.5240486
TGGGAGA33300.024.3873715
CGATCTG98650.023.5409975
CGATCTA56400.020.2090195
GATCTCA27250.019.5205156
GATCTGG40950.019.4847666
CTAGGAC49400.019.3233413
TAGGACT49100.019.247964
ATGCTTG33150.019.1982236
TCTAGGA52100.019.051172
Produced by FastQC (version 0.11.2)