FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s41.352000000d804a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s41.352000000d804a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3382
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.29568302779420463No Hit
CATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTGGTCGCCGT100.29568302779420463Illumina Single End PCR Primer 1 (100% over 23bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.26611472501478417No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.26611472501478417No Hit
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT80.2365464222353637Illumina Single End PCR Primer 1 (96% over 26bp)
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC70.20697811945594324Illumina Single End PCR Primer 1 (95% over 21bp)
ATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTGGTC70.20697811945594324No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT70.20697811945594324Illumina RNA PCR Primer (96% over 25bp)
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG60.17740981667652278No Hit
CTTATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTG50.14784151389710232No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA40.11827321111768185No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT352.4050323E-454.285715
ATACACA502.2650205E-547.54
TATACAC553.9798062E-543.181823
ACACATC500.001396573338.06
Produced by FastQC (version 0.11.2)