FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s40.352000000d8020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s40.352000000d8020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGCTAACCAACCGGCCAAGACAGCACAACCCCCAGCTCACCCACACCA10.9259259259259258No Hit
ATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTTAAACTCTGTCTC10.9259259259259258No Hit
CAGTAAAGCTCCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCATCT10.9259259259259258No Hit
CTTCCGATCTAGCCCGGGGGTGGTGAGCTTGCCTGTAATCCCAGCACTTT10.9259259259259258No Hit
GCTGGAGGAAAAGACATCAACCGAGATTCCGTTAGTAGTGGCGAGCGAAC10.9259259259259258No Hit
TTTCGGAAGAGCACCCCGTCCAGATCGGAAGCTGTCTCTTATACACATCT10.9259259259259258No Hit
CAAATACTCAGCGCCCTATTCAGACTCGCTTTCGCTACGGCTCCACATCT10.9259259259259258No Hit
CTGGAGGTCCGAACCAGTGCCCGTTGAAAAGGTCTTGGATGACGTGTGGC10.9259259259259258No Hit
ATTTCTTTATTGAGCTAGCTAGGATAATGTTGAATAGAAGAAATGAGAAT10.9259259259259258No Hit
GCTGGAAGGTTAAGAGGAGTGGTTAGCTTCTGCGAAGCTACGAATCGAAG10.9259259259259258No Hit
CTCAGGATACAAAATCGATGTGCAAAATTCACAAGCATCGCTTTACACCA10.9259259259259258No Hit
GGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAA10.9259259259259258No Hit
TTGCCTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCG10.9259259259259258No Hit
AGTTTACTGTTAAGACTACAACGGTATCTAATCGTTTTCGATACCCTAAC10.9259259259259258No Hit
GTACATCCCATGGATATATGTTTTGCCGTTGGGAGAGGGTCACTTTCGAG10.9259259259259258No Hit
TCGCTCTTTCGATCCGCTATCGGGGACGTGTGCTCTCCCGATCCCGTCTC10.9259259259259258No Hit
TCGCATCCTGGGGCTGAAGTCGGTCCCAAGGGTTGGGCTGTTCGCCCATT10.9259259259259258No Hit
GATCTGAACAGGGGGAACTCGGCAAAATGGCCCCGGAACTTCGGGAGAAG10.9259259259259258No Hit
TGTCTAAAGTAGACGACAAAGCAGGGCGTCGACGCCATGGAGAAAGACGG10.9259259259259258No Hit
GCCCAACTCAATGGCAACTAACAATAAGGGTTGCGCTCGTTACGGGACTT10.9259259259259258No Hit
ACGAAATTATCAACTAATTTCGTTTAAGGTGGGCGAAGGCTATTCCTGTG10.9259259259259258No Hit
GGTCCTAAGCTGAGGCCGACAGGCGTAGGCGATGGATAACAGGTTGATAT10.9259259259259258No Hit
CTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGGCGCCTCCGT10.9259259259259258No Hit
GTGCTCTTCCGATCTCACCGGGGCATTCTACAAAAGGTACGCCATCACCC10.9259259259259258No Hit
GCTGCCGAACGACCCCGTAGGGGAATTCGAGATGAGTATAATTACCCCAA10.9259259259259258No Hit
TATGATGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGC10.9259259259259258No Hit
GTCAAGCTCCCTTCTGCCTTTACACTCTGCGAATGATTTCCAACCATTCT10.9259259259259258No Hit
CTGTAGAACCATGCGGTTCAACATGTTATCCGGCATTAGCCCCGGTTTCC10.9259259259259258No Hit
GTCCTTACTTGAAATTCAAATTTCGACATACACTCTACGCTGTCTCTTAT10.9259259259259258No Hit
GCAGTAAGGAATCTTCCACAATGGGTGCAAGCCTGATGGAGCAATGCCGC10.9259259259259258No Hit
AAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAACCGAAAAAAAATAC10.9259259259259258No Hit
TTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTA10.9259259259259258No Hit
GTTACAGGGCTATCACCTGCTCTGGCCACCCTTCCCAGGGTGTTCACCTT10.9259259259259258No Hit
ATCGTTGACAGCCTATCAGGTCTTATATCTTATACAAGACTTCAGCTCAC10.9259259259259258No Hit
CTGTCTCAACGAGAGACTCGGTGAAATCATAGTACCTGTGAAGATGCAGG10.9259259259259258No Hit
TTACTTCCCTTGGAATGGGAGATCTCATCTTAAAGGGGGCTTCACGCTTA10.9259259259259258No Hit
CATCCGTCGCTGCATACGAACGTTGCAGAGGGTATCCCGGCAACCTCCCT10.9259259259259258No Hit
TCCCAAAGTCAAGGGCAGGTTACTCACGTGTTACTCACCCGTTCGCCACT10.9259259259259258No Hit
GTCAAGCAAATCCCATAAAGTTGTTCTCAGTTCGGATTGTAGTCTACAAC10.9259259259259258No Hit
AATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATT10.9259259259259258No Hit
GCTTTGATGCAGCCCTAAAAGGGCACCAAGCAGTCCGGGCGCGGTGGTTC10.9259259259259258No Hit
GCTGAGGGCATGGTGGAATTGTCTACAGATCTTAACATCCAGACAGGACT10.9259259259259258No Hit
GTAGCAGCCTGGATGGCACCGCCGGGGCCCACGACCACCTGTTCGGTCAG10.9259259259259258No Hit
GCCCAGGCTGGTCTCGAACTTGTGAGCTCAAGCCATCTGCCTGCCTCAGC10.9259259259259258No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCTGTCTCTT10.9259259259259258No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9259259259259258No Hit
GGGAGCTTGACTGCGAGACAGACAAGTCGAGCAGGGACGAAAGTCGGGCT10.9259259259259258No Hit
GCCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGG10.9259259259259258No Hit
CTTAACAGTAAACTATGCCGACTAGGGATCGGTCGATGTTATCTTTTTGA10.9259259259259258No Hit
CTTATAATGTAGGAAGAATAACACTGATATTCATCATGACTCAATTAATC10.9259259259259258No Hit
CTCCTAGGCAAAAGAAAAAGATATCACGTGTAATTTTTGCTGATCGTCGC10.9259259259259258No Hit
CTGGAAATCTGTCCGAGGATCTGGAGCAGTGTTGTTTTTGATGGGAAAGT10.9259259259259258No Hit
CTCAGGTGCTGATCGTCAGGAAGGCGGGCGCTTTTTACCGGTGCATCGCC10.9259259259259258No Hit
ATTCTACATTAAACCCGCTTCTCTCCACCCCAAGGCACGTCACTTATTCA10.9259259259259258No Hit
ACCTCGGACGCGACCGACGACCCGGGAGTTGTAGAGCCCCTGGGTTGGCG10.9259259259259258No Hit
GGAGTGGAGCCTGCGGCTTAATTTGATTCAACACGGGGAAACTCACCAGG10.9259259259259258No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGGCTCCGATTCGT10.9259259259259258No Hit
CTTCCGATCTGAGTTGGGGGGTGATCGGCCACACTGGAACTGAGACACGG10.9259259259259258No Hit
CATACCGGCAGTCTCACTTCGATGCGCTCCACTGGTCCTGACGGTCCAGC10.9259259259259258No Hit
GCCTGATATTGAAATTCGGCACAGCTTGTACAGGATAGGTAGGAGCCTTT10.9259259259259258No Hit
CAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAGTTGTTCTCAGTTCGG10.9259259259259258No Hit
GTATTCACCGCAGCATGCTGATCTGCGATTACTAGCGATTCCAACTTCAT10.9259259259259258No Hit
ATCCTACTGCGTCCCCCCATCGATTAAAACGATACTAGGTGGTACAGGAA10.9259259259259258No Hit
AGCGCATGTGGAGGTGCGCTCCGGCGAGTTATTCCCACACCTAGACCAAG10.9259259259259258No Hit
ATTCGGAAATCTCTGGATCACAGCTTACTTACAGCTCCCCAAAGCATATC10.9259259259259258No Hit
GCCTAAATACTACCTAGTGACCGATAGTGAACCAGTACAGTGAGGGAAAG10.9259259259259258No Hit
TGTCTATATTTTCGGTGACAGTGGGGTGGGCATCCGCCCGTCTAACATTT10.9259259259259258No Hit
GATTTGACGTCATCCCCGCCTTCCTCCGGTTTATCACCGGCAGTCTTGCT10.9259259259259258No Hit
TCCTTTTTCTGTGGCTTCTTAGTATGTTGTACTGCCTCAGTCTTTCAAAA10.9259259259259258No Hit
GCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCC10.9259259259259258No Hit
CTCCTAAGGCTAAATACTCTCTAGTGACCGATAGTGAACCAGTACCGAGG10.9259259259259258No Hit
GCCCTTTGTATTGTCCATTGTAGCACGTGTGTAGCCCAAATCATAAGGGG10.9259259259259258No Hit
AACAATAAAACAGAAAAACAAAAAGAGAGATAAGAACACTAAAAAAAAAA10.9259259259259258No Hit
ATAAAAAAGATTGCGACCTCGATGTTGGATTAAGATATAATTTTGGGTGT10.9259259259259258No Hit
TATACACATCTGACGCTCCCGACGGATGCTTCTGTGTCCCCCTCCTGTGC10.9259259259259258No Hit
AGTAGAGACAGGGTTTCCCCATGTTGGCCAGGCTGGTCTCGAACACCTGA10.9259259259259258No Hit
TTTGGGCGCCTCCGTTACAATTTGGGAGGCGACCGCCCCAGTCAAACTGC10.9259259259259258No Hit
GTGTTAAGCCTAGTACCCATTAGTTATTCTTCCTGATCCACTCCCTCCTC10.9259259259259258No Hit
CTCTAAGTTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA10.9259259259259258No Hit
CAGTGCTCTACCCCGTGAGGTATTCACTCGAGGCGCTACCTAAATAGCTG10.9259259259259258No Hit
GTGTAAAGGCATAAGGGAGCTTGACTGCGAGACTTACAAGTCGAGCAGGG10.9259259259259258No Hit
GATCTGGAGGGGGGGGACGAACCTTCCACGCTCCAGGAGCGCACGCCGGA10.9259259259259258No Hit
AGACAGACATGTCGAGCAGGGACGAAAGTCGGGACTAGTGATCCGGCACC10.9259259259259258No Hit
CTACTAAGGAGCGTTTGTTGCCTGCCGGAAGTTCGCGTACTACTGACTGT10.9259259259259258No Hit
CCCGTAAACGGGCTCTGACTACTTGTAAGCATACGGTTTCAGGTGCTCTT10.9259259259259258No Hit
GTCTTCCCACCCGGTTAGGGTGGCGAGTTAGAGGGGTCATACCGTTAGGG10.9259259259259258No Hit
CCCGGGAGGGGAGTGAAAGAGCACCTGAAACCGTATGCTTACAAGTAGTC10.9259259259259258No Hit
TAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCG10.9259259259259258No Hit
ATGTAGTCGAGTTGCAGACTACAATCCGAACTTAGAACAACTTTATGGGA10.9259259259259258No Hit
TACCGCTGATTCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAAC10.9259259259259258No Hit
GTCCTCCATTCAGTGTTACCTGAACTTCAACCTGGTCATGGGTAGATCAC10.9259259259259258No Hit
ACGCAAGTCAGCCGCAGTGAATAGGCCCAGGCGACTGTTTACCAAAAACA10.9259259259259258No Hit
TTTTTGGAATCGCCGTAATGATTAATAGGGACAGTCGGGGGCATTAGTAC10.9259259259259258No Hit
TCCATATCTCTGCGCATTTCCCGCTACACATGGAATTCCACTTTCCTCTT10.9259259259259258No Hit
CTTCAACGGCCCTTCAGGAGGATCAAGTCCTCAGGGAAATCTTATCTTAA10.9259259259259258No Hit
ACTCGACTACATGAAGCTGGAATCGCTAGTAATCGTAGATCAGCATGCTA10.9259259259259258No Hit
AGTGAACTCTCGTTAAGGAACTCGGCAAAATGGCCCCGTAACTTCGGGAG10.9259259259259258No Hit
CGACGGCACACGTGCTATGCCGTCGGAAGCCGGGAGGACCATCTCCCAAG10.9259259259259258No Hit
TCACTCTTGTTTTCTCTTCCTACAGCTACTGAGATGTTTCAGTTCACCGC10.9259259259259258No Hit
GTGCCAAACCTCCCCGTCGATGTGAACTCTTGGGGGAGATAAGCCTGTTA10.9259259259259258No Hit
ATTCTAAGGTGAGCGAGCGAACTCTCGTTAAGGAACTCGGCAAAATGACC10.9259259259259258No Hit
GATTTGTATGGCTGGAATGTACCAAATGCCCATCTTAATCTCTCAGTTGA10.9259259259259258No Hit
CACCTGACGCTGCCTTTTGAGCGCATACAAACAATTTAGACATGGCGCCA10.9259259259259258No Hit
ATAAACTTATGATGCTGTCGAAGAAGAACACGGTGGATGAGTAGGAGTGG10.9259259259259258No Hit
TAGCTACCCAGCTCTGCCATAGGCATGACAACTGGTGCACCAGCGGTGCG10.9259259259259258No Hit
CTTTACGCCCAATAATTCCGGATAACGCTTGCCACCTACGTATTACCGCG10.9259259259259258No Hit
GTGTAAAGGCAGAAGGGAGCTTGACTGCGAGACAGACAAGTCGAGCAGGG10.9259259259259258No Hit
TCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTC10.9259259259259258No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers