FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s4.352000000d7b85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s4.352000000d7b85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG100.4945598417408506No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC50.2472799208704253No Hit
CCCTTAAGCTTAGTTGCCATCATTCAGTTGGGCACTCTAAGTTGACTGCC30.1483679525222552No Hit
AAGTTGTTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTG30.1483679525222552No Hit
CTTTACGCCCAATAATTCCGGATAACGCTTGCCACCTACGTATTACCGCG30.1483679525222552No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT30.1483679525222552Illumina RNA PCR Primer (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)