FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s37.352000000d7fd1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s37.352000000d7fd1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.24410089503661514No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG50.20341741253051263No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC30.12205044751830757Illumina Single End PCR Primer 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACG200.001834983171.258
ATCTGAC200.001834983171.257
TACACAT250.004445450457.02
ATACACA250.004445450457.01
CACATCT300.00914712647.54
AGAGAGG150.00972692647.526-27
CGACGAA250.001463811538.018-19
Produced by FastQC (version 0.11.2)