FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s36.352000000d7fc4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s36.352000000d7fc4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAGTTGTT30.28169014084507044No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG20.18779342723004694No Hit
GTGCCCAACTGAATGATGGCAACTAAGCTTAAGGGTTGCGCTCGTTGCGG20.18779342723004694No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT20.18779342723004694Illumina RNA PCR Primer (96% over 25bp)
CTAGCAAGACTGCCGGTGATAAACCGGAGGAAGGCGGGGATGACGTCAAA20.18779342723004694No Hit
CGTAAGTAACTGTGCGCGTCTTGACGGTACCTAATCAGAAAGCCACGGCT20.18779342723004694No Hit
GCTTAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACG20.18779342723004694No Hit
CCCTTAAGCTTAGTTGCCATCATTCAGTTGGGCACTCTAAGTTGACTGCC20.18779342723004694No Hit
GTTGCAGACTACAATCCGAACTGAGAACAACTTTATGGGATTTGCTTGAC20.18779342723004694No Hit
ACAATGGACAATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCA20.18779342723004694No Hit
CCCTGGGAAGGGTGGCCAGAGCAGGTGATAGCCCTGTAACCGACCGAGCG20.18779342723004694No Hit
GTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGT20.18779342723004694No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)