FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s34.352000000d7f81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s34.352000000d7f81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4157450
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107620.2588605996464179No Hit
GTGTAAAGGCAGAAGGGAGCTTGACTGCGAGACAGACAAGTCGAGCAGGG84410.20303310923763365No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA72870.17527570987023294No Hit
ATTCTAAGGTGAGCGAGTGAACTCTCGTTAAGGAACTCGGCAAAATGGCC49830.11985712395819552No Hit
ATACAAAGGGCAGCGAAACCGCGAGGTCAAGCAAATCCCATAAAGTTGTT49070.11802908032568041No Hit
TCGTGAGACAGTTCGGTCCCTATCCGTCGCGGGCGTTGGAGATTTGAAGA45530.10951424551107049No Hit
CTCTAAGTTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA44860.10790268072977426No Hit
GTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACA44840.10785457431839228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT131700.062.566622
CCGATCT139200.058.9226264
TCCGATC139550.058.842913
CGATCTA26650.053.2847565
CGATCTT30050.048.8363275
CTTCCGA175600.047.4658851
CGATCTC47450.046.1417165
CGATCTG76500.042.402195
TCTTCCG61850.036.85795
GATCTGC25500.034.6439866
GATCTAG14350.034.0910576
GATCTCT20550.033.2817236
CTCTTCC77550.029.8859464
GCTCTTC75750.029.7183513
GTGCTCT80400.028.6493431
GATCTTT16950.028.3013256
TGCTCTT80500.028.0827772
ATCTGGT9250.027.2138047
GATCTGA25250.027.0867086
GATCTTC15500.026.9653536
Produced by FastQC (version 0.11.2)