FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s33.352000000d7f67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s33.352000000d7f67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3949667
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT122010.3089121184140334No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA107690.27265589731995127No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTT101300.2564773182144216No Hit
CTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTC87540.2216389381687114No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT87260.22093001764452547No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAG85970.21766391951524014No Hit
CTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTC78690.19923198588640512No Hit
AGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAA78310.19826987946072416No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA72840.18442061064894838No Hit
GTCTATTGTCTATTGTCTATTGTCTATTGTCTATTGTCTATTGTCTATTG59760.1513038947334041No Hit
ATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTT47880.12122540963579967No Hit
AGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGA47750.12089626796385619No Hit
ACATAGAATAACATAGAATAACATAGAATAACATAGAATAACATAGAATA43210.1094016280359838No Hit
ATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCT43160.1092750350852363No Hit
ACAATAGACAATAGACAATAGACAATAGACAATAGACAATAGACAATAGA42370.10727486646342589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT46050.045.3780864
TCCGATC50900.041.707383
TTCCGAT51200.041.2774852
CTTCCGA57850.037.517681
CGATCTG32800.029.3930975
CGATCTA16200.025.5051235
CGATCTC21350.021.354855
TCTCGGG18050.020.53078394-95
TCTTCCG30350.019.8732195
GATCTGG22300.019.1680226
GGTCGCC123850.018.9504394-95
GGTGTAA36250.018.87307494-95
CGATCTT18400.018.0677225
GTATATA29300.017.5056931
TCGCCGG21850.017.3950994-95
GTGCTCT36000.017.281921
GGGAGAC13300.017.1407266
TGGTCGC135600.016.800394-95
GATCTGC19350.016.6904346
GTCTTAC13100.016.6766851
Produced by FastQC (version 0.11.2)