FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s30.352000000d7f24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s30.352000000d7f24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6610541
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1392092.1058639527385123No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102260.15469233153534637No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT95240.14407292837303332No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91330.138158132594594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGACG220700.043.2537427
GAGACGT212750.042.0802548
GGGAGAC235750.041.6807446
CCGATCT484500.039.0513274
TCCGATC502800.038.3192253
CGATCTG198700.038.2173775
TTCCGAT505050.038.2143362
AGACGTG216700.037.5435189
CGATCTA98600.035.6422465
AGGGAGA114350.034.553935
TGGGAGA103800.033.4906855
CAGGGAG66350.032.4240724
CTTCCGA654000.032.2267531
CGATCTT143700.032.0241245
GATCTGG81700.030.3441586
GATCTAG39950.028.4123946
TCCGAAC57850.028.4040133
AGACGTT29450.028.3833689
CTGGGAG70250.026.9058674
GATCTTT98800.025.9094726
Produced by FastQC (version 0.11.2)