FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s3.352000000d7b78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s3.352000000d7b78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7652
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.5880815473078934No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT360.4704652378463147Illumina RNA PCR Primer (96% over 25bp)
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG230.30057501306847884No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC220.2875065342394145No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.23523261892315736No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.19602718243596443No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.15682174594877157No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC156.367319E-495.06
TCCCTAC205.22222E-447.50000480-81
TTATACA952.8921932E-1045.02
TATACAC1004.783942E-1042.7500043
CTTATAC951.535409E-840.01
ATCATTA307.9133315E-539.58333650-51
CGCTGCC1707.094059E-1130.7352968
CTGACGC1707.094059E-1130.7352964
GACGCTG1401.2876626E-830.5357116
TCTGACG1751.0004442E-1029.8571413
TGACGCT1451.812623E-829.4827585
ACGCTGC1651.879016E-928.7878787
GCTGCCG1651.879016E-928.7878789
CACATCT1502.5214831E-828.4999987
CATCTGA1851.9281288E-1028.2432441
ACATCTG1353.378882E-728.1481488
CATTAAA604.9414302E-627.70833452-53
ATCTGAC1902.6193447E-1027.52
TACACAT1404.645699E-727.1428552
TTAAAAA705.203292E-727.14285554-55
Produced by FastQC (version 0.11.2)