FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s26.352000000d7e75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s26.352000000d7e75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171.486013986013986No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC50.4370629370629371No Hit
CATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTCGCCGT50.4370629370629371Illumina Single End PCR Primer 1 (100% over 23bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17482517482517482No Hit
GTCGTCGGTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAA20.17482517482517482No Hit
ACTCGGGCGTCATGACGTCGCCGATCTTGCGGCTCATCAGGTCGTGATAG20.17482517482517482No Hit
GATATAAGGGAATGCCATTTTGTGGACGGAGTATAGGCAATGGCCTATTT20.17482517482517482No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17482517482517482No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)