FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s25.352000000d7e5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s25.352000000d7e5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA382.260559190957763No Hit
CATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTCGCCGT80.4759071980963712Illumina Single End PCR Primer 1 (100% over 23bp)
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC70.41641879833432477No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG40.2379535990481856No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1189767995240928No Hit
CATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTAGCCGT20.1189767995240928Illumina Single End PCR Primer 1 (95% over 23bp)
CACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTCGCC20.1189767995240928Illumina Single End PCR Primer 1 (100% over 21bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1189767995240928No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)