FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s24.352000000d7e32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s24.352000000d7e32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGACGCTAAAGAAGAAACGCAAAGCATTGGGATTATCATAAAACGC10.6711409395973155No Hit
CAGCCATACCCCCCGCCCTCCCCCCCCACCCCCTCTGCCCCATCTGATAG10.6711409395973155No Hit
ATCCATTGCTATTGTTGGTCTGCAAGCGGCTTATGCTAATTTGCGTACTG10.6711409395973155No Hit
CTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCT10.6711409395973155No Hit
GAGGAGTGGTTAGCTTCTGCGAAGCTACGAATCGAAGCCCCAGTAAACGG10.6711409395973155No Hit
GTTTCGTGATCGGGATCGCGTGGGCAAAGGTTTTCGAATCACTGGATTGG10.6711409395973155No Hit
TTTACAGCTACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAA10.6711409395973155No Hit
CTTCCAAGGGGGGGCCCCTCTCTTTACCGAATTCAGACAAACTCCGAATG10.6711409395973155No Hit
TGTTTATTAAAAACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGG10.6711409395973155No Hit
GGCTAGGGTCGTCCCTATAGGAACATTTCGAGCTGTTTTTGAACCATTTT10.6711409395973155No Hit
TCACAGATCCCCGAGCTCCAAAGGGAGCTTGAGGCCTACCTGGACCACTA10.6711409395973155No Hit
GTGTATGGTATGGTTGGGAAAACAAGTTGAACAAGTCAAAGTACTCGGCG10.6711409395973155No Hit
GATCTTTCCCGGGAAGTTCGCTTTCCTAAGTACTAGTGCCGCAGGAGTTA10.6711409395973155No Hit
AAATATACAATAGACAAAATAGACAATATACAATCTGTCTCTTATACACA10.6711409395973155No Hit
CTGTCTCAACGAGAGACTCGGTGAAATCATAGCTGTCTCTTATACACATC10.6711409395973155No Hit
TTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGACCTATCCT10.6711409395973155No Hit
CGCTTGGGCCTATTCACTGCGGCTCTTCTGGGCTTGCACCCTAAAGAGCA10.6711409395973155No Hit
CGATGGCCCCAGGAAGGAGTGGGTGCGATCGACGACCACGGGAATGCGGT10.6711409395973155No Hit
GTCTGAAAGGTCCGGATAGTCGTCCACATTCGTGCAGAAGTTTCTAAGGT10.6711409395973155No Hit
GATTAGCCTAGCCCAGGAACCCTTGGTCATCCGGAGGACATGTTTCCCAC10.6711409395973155No Hit
GATCGGCACCTTACGAGAAATCAAAGTTTTTGGGTTGGGGGGAGTATGGT10.6711409395973155No Hit
CACAACACACCAAAATTGATAGGACACAGCAAAAACAGTACTAAGAGAGA10.6711409395973155No Hit
CAGGTATTCAAGATGAGAATTCTAAGGTGAGCGAGCGAACTCTCGTTAAG10.6711409395973155No Hit
ATGCTCGTCGGATTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGA10.6711409395973155No Hit
ACGTAGTTCTGTTCCCGCAACTCGTTAAATTGGCGCTCCAAGCTCAGCAA10.6711409395973155No Hit
AGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAA10.6711409395973155No Hit
GGGTGTAGGCGATGGACTTTTTCAGGTTGTCGAAGATCAGGCGACCCTCC10.6711409395973155No Hit
CTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGA10.6711409395973155No Hit
CTTTAAATCCTTTAACAAGGTCCAATTGGAGGGCAAGTCTGGTGCCAGCA10.6711409395973155No Hit
GCGTGGCTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTC10.6711409395973155No Hit
ATGAACATATGGTAGAAGTGAGGACACAGTTGTTGCAGCCAGCAGATGAA10.6711409395973155No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTA10.6711409395973155No Hit
GTCTGTTACTGGGGAATTATCTCATGTGTTAATCTAAAAAGATGTTTTTT10.6711409395973155No Hit
CGTCTAAGCAGTTAGATTGAGTGTTAGGCAAATCCGGCACTCTTAAGATT10.6711409395973155No Hit
ATGGAAGACAATCCACAGAAAATAACTAAACAGAAGTACTGAGGAAGAGA10.6711409395973155No Hit
CCATCACCCATTAACGGGCTCTGACTACTTGTAAGCATACGGTTTTTTGG10.6711409395973155No Hit
TGAACACTCATCCTTAATACCTTTCTTTTTGGGGTAATTATACTCATCGC10.6711409395973155No Hit
GACCATACTCCCCCAAGAACCCAAAAACTTTGATTTCTCGTAAGGTGCCG10.6711409395973155No Hit
TCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGC10.6711409395973155No Hit
CAAGTAAGCTCTGGCTGCAGCAGGATTCCGGCGTGGCGCAGGTTCCGCCT10.6711409395973155No Hit
GTGCCATATGATTGCCGAGGTTCTGGCTCGCAGCGGCGTTTACGTGCGTC10.6711409395973155No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA10.6711409395973155No Hit
AAGCCGTTCTCAGCCATGATGACGAAGTACACAAAGAAGCCGGCGGCGGC10.6711409395973155No Hit
CTTCCAGACCCGCGTCAAGTTCGAGTCCGTCTACAAGGAGGGCAAGAACA10.6711409395973155No Hit
CAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACT10.6711409395973155No Hit
ATTCAGGACTAGGAGCATTTTGGTTCTGCAGGATGTGGGATAGAGGCATG10.6711409395973155No Hit
GGTCTTTCGGCGTGGGTGTTTTTCACACCCATTGTCGTTACTCATGTCAG10.6711409395973155No Hit
CTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGA10.6711409395973155No Hit
GAGTCGTTGATTTCACACTGCCGAGAAAAAGCCTCTAGATAGAGATTAGG10.6711409395973155No Hit
CCTGACGGCTGCACCCAACTGCAAGGAAAACACGTGTTCTCAATTGGTGG10.6711409395973155No Hit
GGTTAATTGATGGGGTTAGCGTAAGCGAAGCTCTTGATCGAAGCCCCAGT10.6711409395973155No Hit
AGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAA10.6711409395973155No Hit
TCCCAGTACAGATCGGAAGAGCACACGTCTCCCAGAACAGATCGGAAGAG10.6711409395973155Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACAAGGATCTGCAGGCCTATGAAATCGACTACGCCCGCTTTAACGAAACC10.6711409395973155No Hit
TCTTTGGGCCAACAGGGCTGAAGACTTTGGATTTGACCACATTCGCTCGG10.6711409395973155No Hit
TGTTGATTCCATTCCTTTCCATTCCATTCGGTTTGAAGGCATTCCATTCC10.6711409395973155No Hit
TATTCCCCATCCCATTTACTTCGACATGATCCCAGTTATCTTTCTCACTA10.6711409395973155No Hit
TGAAACCTCGTCCGTGGAGTCCAACGTACGTGTATATCGTCGTCTTTCAC10.6711409395973155No Hit
TAAGATATGGTGATTATGACGTGGTCATTCAACGATAACACTTCTACTAT10.6711409395973155No Hit
TAACGGGGCCGCGCTGAAAGGAAAAAGGAAATCTTCGAATTTAAAAGGAA10.6711409395973155No Hit
CAGAAGATCTTGGCATAGCAGGCAATGATAACCAGGCAAGGGATGACGAA10.6711409395973155No Hit
GTTAAGACTACAACGGTATCTAATCGTTTTCGATACCCTAACCTTCGCCC10.6711409395973155No Hit
GAAGCCCACGCCAGATCGGAAGAGCACCTGTCTCTTATACACATCTGACG10.6711409395973155No Hit
CGTATAGGGTGTGATGCCTGCCCGGTGCTGGAAGGTCTGTCTCTTATACA10.6711409395973155No Hit
AGCGAAAAGCGTCCAAAGACAGTGAGGAGAAACTAAAATATGCCCGCGAT10.6711409395973155No Hit
GGATAAAAGCTACCCCGGGGATAACAGGCTTATCTCCCCAAGAGTTCACA10.6711409395973155No Hit
GTTGTCTTCGGACATGTTGCCCAGCGAGGCACCGATGCCGCCTTGGGCCG10.6711409395973155No Hit
GCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCG10.6711409395973155No Hit
ATATTATACAATAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTT10.6711409395973155No Hit
TATTTCTCACGGGCCGAAAAATTATCATGATAAAGCAGGAATTACTACTG10.6711409395973155No Hit
CTCATGTGCATGTCATCCATCGATAGTTCCACATTTGATATGCCATCAGA10.6711409395973155No Hit
ATATGCAGCTGCAAGTTAAGCACGAGAAGATAGTCCTTTATCAGCGGGAG10.6711409395973155No Hit
GAGATTAGGTGCCCGTACCGCAAACCGACACAGGTAGTCAAGATGAGAAT10.6711409395973155No Hit
CTCTATATAGAGAGGGTATCGGGATCCCGGGTGTTTTGGGGCCAGTTTCC10.6711409395973155No Hit
GGTCAGCTCGGCGCCGGCCTCGGGGTCGCGGGTCAGGTTCTCGCCGGTCT10.6711409395973155No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6711409395973155No Hit
TTCCAGCACCGGGCAGGCGTCAGCCCCTATACATCACCTTACGGTTTAGC10.6711409395973155No Hit
CATCTAAGATGTACAAGTGCAAGTTTGTCACCTGTCCACCTTCCACATTG10.6711409395973155No Hit
ACTCTACTTAGTTGGCACCGTCCTTATTGGCCATGAAGGCGATGAGGTCC10.6711409395973155No Hit
GACAAGGTCAAAGAGAAGGAAAAGTCCCTGGAAAGCGAAAAGCTCCTCAT10.6711409395973155No Hit
CCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTG10.6711409395973155No Hit
CCCCTTCTCCCGAAGTTACGGGGTCATTTTGCCGAGTTCCTTAACGAGAG10.6711409395973155No Hit
GCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGA10.6711409395973155No Hit
TATCAGCCCTCGCAAACCATATCCGTCCCATATCCCACCAGACAGCCCGT10.6711409395973155No Hit
TCCCTTTACTGTGGAAGGATGAACGGGTTGCAATAAACTGGTTGCTGCGA10.6711409395973155No Hit
CTGCAGAGGGAGGTTCCCTCAGAATGGTTGAAAATCATTCGCAGAGTGTA10.6711409395973155No Hit
GAAATTAATTGTTTAAACTCGTAATTTATTGGCCCATGCCGAACTCGATT10.6711409395973155No Hit
GGGCTAGCCTCAAGTGATGATTATTGGAGGTAGAGCACTGTTTGGACGAG10.6711409395973155No Hit
CATCTTACGCTTCCCAAGAATAGACGAGACTCGAGCACTAGACTCCCCCT10.6711409395973155No Hit
ACCTAGCTCCTAGCTTGGACGCGCTAGTCCAGCCTGTCTCTTATACACAT10.6711409395973155No Hit
ACCTTACTCCTTGACCCCGGTCATGGACCGCGCCCGGCCTCGCCCCCACC10.6711409395973155No Hit
ATACAAAGGGCAGCGAAACCGCGAGGTAAAGCAAATCCCATAAAGTTGTT10.6711409395973155No Hit
GACGTGTGCTCTCCGTTCTCATTAGGGAGACGTGTGCTCTCCGTTCTCAT10.6711409395973155No Hit
CATCTGGCGCTTTCGCCGCCGTGGGTCGTGTTCTTCCCTGCGGTCGGCGT10.6711409395973155No Hit
GTAATAGTACCGGCCCACAATAACACTCGTTTAAGAGCACTAATGCAGGT10.6711409395973155No Hit
GCACTCCAATGACCACAATGCCATCTTTAAAGTTGGCCGCCATTGTCATG10.6711409395973155No Hit
GAGAGAGAGAGAAAGAGAAAGAGACAGGTGGAGAAATGGCGAAAGAGAGG10.6711409395973155No Hit
ATCCCATACCCTTCGGGGCCATCTTCGGCTACCTGGCGCTTTTGCTCCTG10.6711409395973155No Hit
ACCCAACACGAGTTATGTCGTGTTATCGACATGTGAATTCATAGCATGTC10.6711409395973155No Hit
CCGTAAGGTGATGTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAA10.6711409395973155No Hit
AAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGCTGTCT10.6711409395973155No Hit
GAAGGGCCATCGCTCAACGGATAAAAGCTACCCCGGGGATAACAGGCTTA10.6711409395973155No Hit
ACTAGAGACTCTTCACCTTGGAGACCAGCTGCGGATATTGGTACGGCCTG10.6711409395973155No Hit
GAACTAGGGCGGTATCTTATCGCCTTCGAACCTCTAACTTTCGTTCTTGA10.6711409395973155No Hit
TCCCGTACACGTATAAGCGCGCCCATGGTGAATCAGTGATACTAACTGTC10.6711409395973155No Hit
TTTCCCCCCTTCCCTCAAGGCAGACAACCATCAGATAACGAGCCATCTAC10.6711409395973155No Hit
ACGCAAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTG10.6711409395973155No Hit
CCCAAGAGAGATCGGAAGAGCACACGTCTCCCAAGAGAGATCGGAAGAGC10.6711409395973155Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT10.6711409395973155No Hit
CCCAAAGCCATTCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAA10.6711409395973155No Hit
GCTGGCAACTGTTGGTGGCCGGCTTCTTGGTGTCCTTGGCCTGTCTCTTA10.6711409395973155No Hit
GAGGTGATTTATGTTTGGTACTATTGCTGGCGGTATTGCTTCTGCTCTTG10.6711409395973155No Hit
CTCCGTATAGAGTGTCCTACAACCCCAATAAGCAAGCTTATTGGTTTGGG10.6711409395973155No Hit
CCCTAAAGAGCACCCCTTCTCCCGAAGTTACGGGGTCATTTTGCCGAGTT10.6711409395973155No Hit
ATATCCTGTGATCCGGCGATATCTGAATGGGGAAACCCACTTGTCATAAG10.6711409395973155No Hit
CCCCTATACATCACCTTACGGTTTAGCAGAGACCTGTGTTTTTGATAAAC10.6711409395973155No Hit
AGCACTCGTGACGCCCACTCCAGAACCCCTCTCCATCGCCCCACTCAACA10.6711409395973155No Hit
TCTTCCGAACACATACGGGGTGTGCTGCTTCCAAAAAAAAAAAAAAAAAA10.6711409395973155No Hit
CCTGGCAGCAGCTTTTGATTTCGCCACATTGACGTGAGACGCAGCAGGCG10.6711409395973155No Hit
TACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAA10.6711409395973155No Hit
TTTTTGTCCATTTTTGACCGGTTGGTTCACAAATCGCAGAATAATTTGAT10.6711409395973155No Hit
GTCTGCAGGTATTCGCGCAGATAGGCGGCCACGTCGAAGCCGGTCAGCCC10.6711409395973155No Hit
TCTCTGCGCATTTCACCGCTACACATGGAATTCCACTTTCCTCTTCTGCA10.6711409395973155No Hit
GCTCTAATTTGTCTAGGAAATAACCGTCAGGATTGACACCCTCCCAATTG10.6711409395973155No Hit
CTTCTTGATGGAGGCGTACGGTTGGTTCGGCACTGCTTACTTTATGTACG10.6711409395973155No Hit
GGGGGAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGAT10.6711409395973155No Hit
GTGAGATTGAGTGTTAGGCAAATCCGGCACTCTTAAGATTGAGCTGTGAT10.6711409395973155No Hit
ATCTTCCTGACACCCCAGGGTCGCCAACTGCGCAACCAGGCGCTGGACAT10.6711409395973155No Hit
AGCAAATGCAAAAACTGAAATTACTCGATTTTCTTTTTTGTTAAATCATG10.6711409395973155No Hit
GCTTCCATGGTTGTAATCACATAAACAGCAGAGGATTAACAGCAGAACAA10.6711409395973155No Hit
GCCGAATACATCTTGGCCCTTAATGCTGCTGACGCCACTGCTGCTGTCGA10.6711409395973155No Hit
GTTCTACGAGCAGAGCTTCGGCAAGTCCCTAGAGTCCGACCTAAAGGGCG10.6711409395973155No Hit
GTAGCAGGCAGGCCCCAGGCAGAGGCCAAGAGGAGGGGAACTGCAGCTGG10.6711409395973155No Hit
CATTAGTACTCACATGCTAGAGGTGAAATTCTTGGATTTTGTGAAGACCT10.6711409395973155No Hit
TCTTATCACTCGCAGTGTGACTCCCGGACTGGCATGGATGGCATTCGGAG10.6711409395973155No Hit
GGTTCCTCCGCACAGTTAATAGGCGTGGGGTCAGTAGGACCCTAAGGCGT10.6711409395973155No Hit
TACAAGGGAGACGTGTTCCCTTCCGATCTTAACAGGGAGACGTTTGCCCT10.6711409395973155No Hit
GAGCTGTCCTTAGTACGAGAGGACCGGGATGGACATACCTCTGGTGTACC10.6711409395973155No Hit
ATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGG10.6711409395973155No Hit
CTATTAAACACCCACACTGCTACACTCCCACACACACAGCCACATGCCCA10.6711409395973155No Hit
GTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTC10.6711409395973155No Hit
TGATTCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCG10.6711409395973155No Hit
CATTCGCGGTGGCGGTCACTGCATCTACAGCTACATGTTCATGATGGAGT10.6711409395973155No Hit
ATCTAACATGGGGTGTGCTCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAA10.6711409395973155No Hit
GGGTTGCTTCAGACAAAGGGGGCTCAGCAAGTAGAGGATTGGATCAATCA10.6711409395973155No Hit
CTGTTCTTCTTCAGGTACTTCTTGGTCAAGTACTTGAGGTATGCCTTGGA10.6711409395973155No Hit
GACCAAAAGGGCAGCAGAAACAACCAGGTCCTGCTGTTGCTGCACGACAG10.6711409395973155No Hit
CACGTACCCATTCGGGGTGTTTCAGTTGGAGCTGATTTTGCTTTTGATGC10.6711409395973155No Hit
CTCTAATCTCTGGGCATCTGGCTAGGATGTTGATGGAACTGACCAAACGT10.6711409395973155No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers