FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s22.352000000d7dfd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s22.352000000d7dfd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4849694
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1077532.221851523003307No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95710.19735265771407434No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92300.1903212862502253No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT90520.1866509515858114No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83650.17248510936978703No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT68170.14056556970398545No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA63150.1302144011560317No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG62750.12938960684942186No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT51290.10575924996504935No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA51240.10565615067672311No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG50670.10448081878980406No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC49400.10186209686631775No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC164850.044.251976
GGAGACG171750.041.064737
GAGACGT166650.040.440488
AGGGAGA81550.037.6201865
TGGGAGA69350.036.5000235
AGACGTG177500.035.3195239
CAGGGAG50100.030.9024034
TAGGGAG27200.030.0311474
TTCCGAT283850.028.1081832
CCGATCT281850.028.088594
TCCGATC294250.027.1308633
AGACGTT18700.026.1598289
GTAGGGA12900.026.1385233
TCCGAAC32350.023.4886513
CGGGAGA49850.023.24545
CTTCCGA382500.023.2427121
CTAGGGA13850.022.6311473
TATGCTT35700.021.550595
CTAGGAC49200.021.3323923
TCTAGGA52100.021.1476762
Produced by FastQC (version 0.11.2)