FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s21.352000000d7dc6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s21.352000000d7dc6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA251.0964912280701753No Hit
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT130.5701754385964912Illumina Single End PCR Primer 1 (96% over 26bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.5263157894736842No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG70.30701754385964913No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTT40.17543859649122806No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC30.13157894736842105No Hit
CATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTCGCCGT30.13157894736842105Illumina Single End PCR Primer 1 (100% over 23bp)
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG30.13157894736842105No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13157894736842105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA250.004396142457.02
TACACAT250.004396142457.05
TATACAC300.00904621747.53
ACACATC300.00904621747.56
CTTATAC300.00904621747.51
ATACACA300.00904621747.54
Produced by FastQC (version 0.11.2)