FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s2.352000000d7b5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s2.352000000d7b5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7136
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT510.7146860986547086Illumina RNA PCR Primer (96% over 25bp)
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG410.5745515695067265No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC240.336322869955157No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.32230941704035876No Hit
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT160.2242152466367713Illumina Single End PCR Primer 1 (96% over 26bp)
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG150.2102017937219731No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.1961883408071749No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.18217488789237668No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.15414798206278027No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC90.12612107623318386Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGG90.12612107623318386Illumina RNA PCR Primer (95% over 24bp)
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGC80.11210762331838565Illumina RNA PCR Primer (95% over 24bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCC156.348017E-495.03
CGTATCA250.001562652438.048-49
GCTGCCG1850.035.9459469
CTTATAC1202.7921487E-935.6251
CGCTGCC1900.035.0000048
GGTCGCC501.1836764E-633.2542-43
GACGCTG2050.032.4390266
ACGCTGC1950.031.6666687
GTGGTCG452.2506809E-531.66666840-41
TGGTCGC404.276444E-429.68750240-41
GTCGCCG404.276444E-429.68750242-43
TATACAC1302.3843677E-729.230773
TTATACA1153.203304E-628.9130442
CACATCT1353.319219E-728.1481487
TGACGCT1953.43789E-1026.7948725
ACATCTG1256.132459E-626.5999988
AGATCTC458.518177E-426.38889132-33
TACACAT1456.2035906E-726.20695
ATACACA1456.2035906E-726.20694
ATCTCGG558.846966E-525.90909234-35
Produced by FastQC (version 0.11.2)