FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s19.352000000d7d76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s19.352000000d7d76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3249
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA331.0156971375807942No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.2770083102493075No Hit
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT70.21545090797168356Illumina Single End PCR Primer 1 (96% over 26bp)
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG40.12311480455524776No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCG200.001889588371.259
TCGGTGG150.00986582647.536-37
CTCGGTG150.00986582647.536-37
ATCATCA150.00986582647.550-51
Produced by FastQC (version 0.11.2)