FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s18.352000000d7d5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s18.352000000d7d5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6735010
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA235550.3497396440391328No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA80830.1200146696144475No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA75630.11229381990524143No Hit
GTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGGTTA75170.11161082166173474No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT261250.047.0111352
CCGATCT268800.045.1613124
TCCGATC273050.044.7534073
CTTCCGA337050.038.3268471
GAGACGT87800.036.1354188
GGAGACG96350.035.2949377
CGATCTG147950.035.1188745
GGGAGAC102600.033.885326
CGATCTA75350.031.830715
AGACGTG92550.031.2016989
CGATCTC91900.027.958895
GATCTAG30100.026.9822086
GATCTGG74950.024.9673486
AGGGAGA57350.024.6786295
CGATCTT100650.021.753315
GATCTCA43200.020.1194086
TCTTCCG192900.019.2290135
TGGGAGA65650.019.0988565
TCTAGGA45850.018.2306372
CTAGGAC43450.018.1445753
Produced by FastQC (version 0.11.2)