FastQCFastQC Report
Mon 14 May 2018
HG5M3BCX2_l01n02_r3zt2s17.352000000d7d33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG5M3BCX2_l01n02_r3zt2s17.352000000d7d33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA441.2998522895125553No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC70.206794682422452Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT60.1772525849335303Illumina Single End PCR Primer 1 (96% over 26bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14771048744460857No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14771048744460857No Hit
CATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTCGCCGT50.14771048744460857Illumina Single End PCR Primer 1 (100% over 23bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14771048744460857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)